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首页> 外文期刊>Molecular and Cellular Biology >Induced differentiation of avian myeloblastosis virus-transformed myeloblasts: phenotypic alteration without altered expression of the viral oncogene.
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Induced differentiation of avian myeloblastosis virus-transformed myeloblasts: phenotypic alteration without altered expression of the viral oncogene.

机译：诱导禽成纤维细胞病病毒转化的成纤维细胞的分化：表型改变而病毒癌基因的表达没有改变。

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Cells of a clone of avian myeloblastosis virus-transformed myeloblasts were induced to differentiate to adherent myelomonocytic cells by treatment with lipopolysaccharide. These adherent cells were subcultured and maintained as a line for more than 6 months with lipopolysaccharide present. Cells of this line were induced to differentiate to nondividing macrophage-like cells by the addition of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. In this way, the following homogeneous cell populations representing three distinct stages of myeloid differentiation were obtained: I, actively dividing myeloblasts that grew in suspension: II, actively dividing adherent cells; and III, fully differentiated nondividing cells resembling macrophages. When the expression of v-myb (the oncogene of avian myeloblastosis virus) was examined in cells of these three differentiation stages, it was found that the protein encoded by v-myb (p45v-myb) continued to be synthesized in similar quantities and showed no obvious alteration (assessed by partial proteolytic digestion and two-dimensional gel electrophoresis) during differentiation. These results show that cells transformed by v-myb can be induced to differentiate without affecting the expression of v-myb and imply that, during differentiation, the effect of v-myb is suppressed by a mechanism other than altered expression of the oncogene.
机译：通过用脂多糖处理，诱导了禽成纤维细胞病病毒转化的成纤维细胞的克隆的细胞分化为粘附的成肌细胞。将这些贴壁细胞传代培养，并在存在脂多糖的情况下作为品系维持6个月以上。通过添加肿瘤启动子12-O-十四烷酰佛波醇-13-乙酸酯，诱导该细胞系分化为不分裂的巨噬细胞样细胞。这样，获得了代表骨髓分化的三个不同阶段的以下均质细胞群：I，活跃地分裂成悬浮液的成肌细胞：II，活跃地分裂贴壁细胞; III，完全分化的非分裂细胞，类似于巨噬细胞。在这三个分化阶段的细胞中检查了v-myb（禽成纤维细胞病病毒的致癌基因）的表达后，发现由v-myb编码的蛋白（p45v-myb）继续以相似的数量合成并显示出分化期间无明显变化（通过部分蛋白水解消化和二维凝胶电泳评估）。这些结果表明，可以通过v-myb转化的细胞被诱导分化而不影响v-myb的表达，这暗示在分化期间，v-myb的作用被癌基因表达改变以外的机制所抑制。

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