Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein.

J C Lacal; S A Aaronson

首页> 外文期刊>Molecular and Cellular Biology >Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein.

【24h】

Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein.

机译：单克隆抗体Y13-259识别不直接参与蛋白质GTP结合活性的p21 ras分子的表位。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The p21 products of ras proto-oncogenes are GTP-binding proteins with associated GTPase activity. Recent studies have indicated that ras p21 may be required for the initiation of normal cell DNA synthesis, since microinjection of a monoclonal antibody, Y13-259, blocks serum stimulation of DNA synthesis in quiescent cell cultures (L. S. Mulcahy, M.R. Smith, and D. W. Stacey, Nature [London] 313:241-243, 1985). We localized the structural domain within the p21 molecule recognized by the Y13-259 monoclonal antibody. By analysis of a series of bacterially expressed p21 deletion mutants, the monoclonal antibody was found to interact with a region between positions 70 and 89 in the p21 amino acid sequence. By comparison of the coding sequences of different p21 proteins recognized by this monoclonal antibody, a highly conserved amino acid region between positions 70 and 81 was found to be the most likely site for the epitope detected by the Y13-259 antibody. This monoclonal antibody was further shown not to interfere directly with in vitro biochemical functions of the molecule, including GTP binding, GTPase, and autokinase activities.

机译：ras原癌基因的p21产物是具有相关GTPase活性的GTP结合蛋白。最近的研究表明，可能需要ras p21来启动正常细胞DNA合成，因为显微注射单克隆抗体Y13-259会阻止静态细胞培养物中血清对DNA合成的刺激（LS Mulcahy，MR Smith和DW Stacey ，自然[伦敦] 313：241-243，1985）。我们将结构域定位在被Y13-259单克隆抗体识别的p21分子内。通过分析一系列细菌表达的p21缺失突变体，发现该单克隆抗体与p21氨基酸序列中70和89位之间的区域相互作用。通过比较该单克隆抗体识别的不同p21蛋白的编码序列，发现在位置70和81之间的高度保守的氨基酸区域是由Y13-259抗体检测到的表位的最可能位点。进一步表明，这种单克隆抗体不会直接干扰分子的体外生化功能，包括GTP结合，GTPase和自身激酶活性。

著录项

来源
《Molecular and Cellular Biology》 |1986年第4期|共8页
作者
J C Lacal; S A Aaronson;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein. [J] . J C Lacal, S A Aaronson Molecular and Cellular Biology . 1986,第4期

机译：单克隆抗体Y13-259识别不直接参与蛋白质GTP结合活性的p21 ras分子的表位。
2. H-ras mutants lacking the epitope for the neutralizing monoclonal antibody Y13-259 show decreased biological activity and are deficient in GTPase-activating protein interaction. [J] . S K Srivastava, A Di Donato, J C Lacal Molecular and Cellular Biology . 1989,第4期

机译：缺少中和性单克隆抗体Y13-259的表位的H-ras突变体显示出降低的生物活性，并且在GTPase激活蛋白相互作用中不足。
3. Direct binding to antigen-coated beads refines the specificity and cross-reactivity of four monoclonal antibodies that recognize polymorphic epitopes of HLA class I molecules [J] . HiltonH.G., ParhamP. Tissue antigens. . 2013,第4期

机译：直接与抗原包被的珠结合可改善识别HLA I类分子多态性表位的四种单克隆抗体的特异性和交叉反应性
4. PEPTIDE PHAGE DISPLAY AND EP1TOPE MAPPING OF A FIBRIL-RELATED CON FORM ATIONAL EPITOPE RECOGNIZED BY THE 11-1F4 MONOCLONAL ANTIBODY [C] . A Allen, S.J. Kennel, A Solomon, International Symposium on Amyloidosis . 2008

机译：肽噬菌体展示和Ep1tope映射的原纤维相关的孔形成到11-1F4单克隆抗体识别的环境表位
5. The potently neutralizing monoclonal antibody 1B7: Its unique epitope, effects on intracellular trafficking, and elicitation upon infection with pertussis. [D] . Sutherland, Jamie Nicole. 2010

机译：强效中和性单克隆抗体1B7：其独特的表位，对细胞内运输有影响，并在感染百日咳后诱发。
6. Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein. [O] . J C Lacal, S A Aaronson 1986

机译：单克隆抗体Y13-259识别不直接参与蛋白质GTP结合活性的p21 ras分子的表位。
7. Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein. [O] . Lacal, J C, Aaronson, S A 1986

机译：单克隆抗体Y13-259识别不直接参与蛋白质GTP结合活性的p21 ras分子的表位。

Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein.

摘要

著录项

相似文献

相关主题

期刊订阅