• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Insertions of up to 17 amino acids into a region of alpha-tubulin do not disrupt function in vivo.
【24h】

Insertions of up to 17 amino acids into a region of alpha-tubulin do not disrupt function in vivo.

机译：在α-微管蛋白区域内插入多达17个氨基酸不会破坏体内功能。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microtubules in yeasts are essential components of the mitotic and meiotic spindle and are necessary for nuclear movement during cell division and mating. The yeast Saccharomyces cerevisiae has two alpha-tubulin genes, TUB1 and TUB3, either of which alone is sufficient for these processes when present in a high enough copy number. Comparisons of sequences from several species reveals the presence of a variable region near the amino terminus of alpha-tubulin proteins. We perturbed the structure of this region in TUB3 by inserting into it 3, 9, or 17 amino acids and tested the ability of these altered proteins to function as the only alpha-tubulin protein in yeast cells. We found that each of these altered proteins was sufficient on its own for mitotic growth, mating, and methods of yeast. We conclude that this region can tolerate considerable variation without losing any of the highly conserved functions of alpha-tubulin. Our results suggest that variability in this region occurs because it can be tolerated, not because it specifies an important function for the protein.
机译：酵母中的微管是有丝分裂和减数分裂纺锤体的基本组成部分，是细胞分裂和交配过程中核运动所必需的。酵母酿酒酵母具有两个α-微管蛋白基因TUB1和TUB3，当它们以足够高的拷贝数存在时，单独使用一个就足以进行这些过程。来自几种物种的序列比较表明，在α-微管蛋白蛋白的氨基末端附近存在可变区。我们通过在TUB3中插入3、9或17个氨基酸来扰动该区域的结构，并测试了这些改变后的蛋白质在酵母细胞中作为唯一的α-微管蛋白的功能。我们发现，这些改变的蛋白质中的每一个都足以实现有丝分裂的生长，交配和酵母的制备方法。我们得出的结论是，该区域可以耐受相当大的变异而不会丢失任何α-微管蛋白的高度保守功能。我们的结果表明，该区域发生变异是因为可以容忍，而不是因为它为蛋白质指定了重要功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号