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首页> 外文期刊>Molecular and Cellular Biology >Regulation of platelet-derived growth factor gene expression by transforming growth factor beta and phorbol ester in human leukemia cell lines.
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Regulation of platelet-derived growth factor gene expression by transforming growth factor beta and phorbol ester in human leukemia cell lines.

机译：通过在人类白血病细胞系中转化生长因子β和佛波酯来调节血小板衍生生长因子基因的表达。

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We studied the expression of the genes encoding the A and B chains of platelet-derived growth factor (PDGF) in a number of human leukemia cell lines. Steady-state expression of the A-chain RNA was seen only in the promonocytic leukemia cell line U937 and in the T-cell leukemia cell line MOLT-4. It has previously been reported that both PDGF A and PDGF B genes are induced during megakaryoblastic differentiation of the K562 erythroleukemia cells and transiently during monocytic differentiation of the promyelocytic leukemia cell line HL-60 and U937 cells. In this study we show that PDGF A RNA expression was induced in HL-60 and Jurkat T-cell leukemia cells and increased in U937 and MOLT-4 cells after a 1- to 2-h stimulation with an 8 pM concentration of transforming growth factor beta (TGF-beta). PDGF A RNA remained at a constant, elevated level for at least 24 h in U937 cells, but returned to undetectable levels within 12 h in HL-60 cells. No PDGF A expression was induced by TGF-beta in K562 cells or in lung carcinoma cells (A549). Interestingly, essentially no PDGF B-chain (c-sis proto-oncogene) RNA was expressed simultaneously with PDGF A. In the presence of TGF-beta and protein synthesis inhibitors, PDGF A RNA was superinduced at least 20-fold in the U937 and HL-60 cells. PDGF A expression was accompanied by secretion of immunoprecipitable PDGF to the culture medium of HL-60 and U937 cells. The phorbol ester tumor promoter tetradecanoyl phorbol acetate also increased PDGF A expression with similar kinetics, but with a mechanism distinct from that of TGF-beta. These results suggest a role for TGF-beta in the differential regulation of expression of the PDGF genes.
机译：我们研究了在许多人类白血病细胞系中编码血小板衍生生长因子（PDGF）A和B链的基因的表达。仅在原单核细胞白血病细胞系U937和T细胞白血病细胞系MOLT-4中才能看到A链RNA的稳态表达。先前已经报道PDGF A和PDGF B基因在K562红白血病细胞的巨核母细胞分化期间以及早幼粒细胞白血病细胞系HL-60和U937细胞的单核细胞分化期间被瞬时诱导。在这项研究中，我们显示了在以8 pM浓度的转化生长因子刺激1至2小时后，HL-60和Jurkat T细胞白血病细胞中诱导了PDGF A RNA表达，而U937和MOLT-4细胞中PDGF A RNA表达增加。 beta（TGF-beta）。在U937细胞中，PDGF A RNA至少在24小时内保持恒定的升高水平，而在HL-60细胞中，则在12小时内恢复到不可检测的水平。 TGF-β在K562细胞或肺癌细胞（A549）中未诱导PDGF A表达。有趣的是，基本上没有PDGF B链（c-sis原癌基因）RNA与PDGF A同时表达。在存在TGF-beta和蛋白质合成抑制剂的情况下，PDGF A RNA在U937和2000中的超诱导倍数至少为20倍。 HL-60细胞。 PDGF A表达伴随着可免疫沉淀的PDGF向HL-60和U937细胞的培养基分泌。佛波酯肿瘤促进剂乙酸十四烷酰佛波酯也以相似的动力学增加PDGF A的表达，但是其机制不同于TGF-β。这些结果暗示了TGF-β在PDGF基因表达的差异调节中的作用。

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