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首页> 外文期刊>Molecular and Cellular Biology >Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes.
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Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes.

机译：顺反子间长度对真核核糖体重新启动效率的影响。

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Simian virus 40-based plasmids that direct the synthesis of preproinsulin during short-term transfection of COS cells have been used to probe the mechanism of reinitiation by eucaryotic ribosomes. Earlier studies from several laboratories had established that the ability of ribosomes to reinitiate translation at an internal AUG codon depends on having a terminator codon in frame with the preceding AUG triplet and upstream from the intended restart site. In the present studies, the position of the upstream terminator codon relative to the preproinsulin restart site has been systematically varied. The efficiency of reinitiation progressively improved as the intercistronic sequence was lengthened. When the upstream "minicistron" terminated 79 nucleotides before the preproinsulin start site, the synthesis of proinsulin was as efficient as if there were no upstream AUG codons. A mechanism is postulated that might account for this result, which is somewhat surprising inasmuch as bacterial ribosomes reinitiate less efficiently as the intercistronic gap is widened.
机译：在猿猴COS细胞的短期转染过程中指导前胰岛素原合成的基于猿猴病毒40的质粒已用于探测真核核糖体的重新启动机制。几个实验室的较早研究已经确定，核糖体在内部AUG密码子上重新启动翻译的能力取决于在前面的AUG三联体和预期的重新启动位点上游具有一个终止子密码子。在目前的研究中，上游终止子密码子相对于胰岛素原前重启位点的位置已经系统地改变了。随着顺反子序列的延长，重新初始化的效率逐渐提高。当上游“ minicistron”在前胰岛素原起始位点之前终止了79个核苷酸时，胰岛素原的合成就如同没有上游AUG密码子一样有效。推测有一种机制可以解释这个结果，这是令人惊讶的，因为随着核糖体间顺反子间隙的扩大，细菌核糖体的启动效率降低。

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