A small nuclear RNA, U5, can transform cells in vitro.

K Hamada; T Kumazaki; K Mizuno; K Yokoro

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A small nuclear RNA, U5, can transform cells in vitro.

机译：小核RNA U5可以在体外转化细胞。

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Low-molecular-weight RNA exhibiting transforming potential was identified in chemically induced lymphoma cells by the transformation of mink lung cells after transfection. The RNA was sequenced by the direct chemical method and was shown to be a small nuclear RNA, U5. The transforming potential of the RNA was further studied in quantitative transformation assays using 3Y1, a rat fibroblastic cell line. Transformed foci appeared with a latency of 3 to 4 weeks after transfection. U5-transformed 3Y1 cells frequently carried an amplified c-myc oncogene. In addition, U5 induced chromosome aberrations in transfected cells, indicating that the RNA acts as a clastogen. Transforming and clastogenic potentials were specifically inactivated when U5 was incubated with RNase H in the presence of a complementary oligonucleotide. We discuss a possible mechanism of U5-induced cell transformation.

机译：通过转染后水貂肺细胞的转化，在化学诱导的淋巴瘤细胞中鉴定出具有转化潜力的低分子量RNA。通过直接化学方法对RNA进行了测序，结果显示该RNA是小核RNA U5。使用大鼠成纤维细胞系3Y1在定量转化试验中进一步研究了RNA的转化潜力。转染后出现病灶，潜伏期为3至4周。 U5转化的3Y1细胞经常携带扩增的c-myc癌基因。另外，U5诱导了转染细胞中的染色体畸变，表明RNA起到了克拉斯托根的作用。当在互补寡核苷酸存在下将U5与RNase H孵育时，转化和产生致胶化的电位被特异地灭活。我们讨论了U5诱导细胞转化的可能机制。

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《Molecular and Cellular Biology》 |1989年第10期|共12页
作者
K Hamada; T Kumazaki; K Mizuno; K Yokoro;
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中图分类细胞生物学;
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1. Conserved Loop I of U5 Small Nuclear RNA Is Dispensable for Both Catalytic Steps of Pre-mRNA Splicing in HeLa Nuclear Extracts [J] . Véronique Ségault, Cindy L. Will, Maria Polycarpou-Schwarz, Molecular and Cellular Biology . 1999,第4期

机译：U5小核RNA的保守环I对于HeLa核提取物中的pre-mRNA剪接的两个催化步骤都是必不可少的
2. Conserved Loop I of U5 Small Nuclear RNA Is Dispensable for Both Catalytic Steps of Pre-mRNA Splicing in HeLa Nuclear Extracts [J] . Véronique Ségault, Cindy L. Will, Maria Polycarpou-Schwarz, Molecular and Cellular Biology . 1999,第4期

机译：U5小核RNA的保守环I对于HeLa核提取物中的pre-mRNA剪接的两个催化步骤都是必不可少的
3. Pseudouridine modification of U5 RNA in ribonucleoprotein particles assembled in vitro. [J] . J R Patton Molecular and Cellular Biology . 1991,第12期

机译：体外组装的核糖核蛋白颗粒中U5 RNA的假尿苷修饰。
4. Identifying Immature Precursor Cells in Bone Marrow Pathological Images Based on Distance Transform and Internal Structures of Cells [C] . Wu Heng, Cao Guitao, Li Ling, 2010 International Conference on Multimedia Technology . 2010

机译：基于距离变换和细胞内部结构识别骨髓病理图像中的未成熟前体细胞
5. SPECIFIC INTERACTION OF BETA GLOBIN NUCLEAR RNA AND ITS PRECURSOR WITH THE NUCLEAR MATRIX IN CHICKEN RED BLOOD CELLS. [D] . ROSS, DAVID ANDREW. 1982

机译：鸡红色血液细胞中β-珠蛋白核RNA的特异性相互作用及其与核基质的前体相互作用。
6. A small nuclear RNA U5 can transform cells in vitro. [O] . K Hamada, T Kumazaki, K Mizuno, 1989

机译：小核RNA U5可以在体外转化细胞。
7. A small nuclear RNA, U5, can transform cells in vitro. [O] . Hamada, K, Kumazaki, T, Mizuno, K, 1989

机译：小核RNA U5可以在体外转化细胞。
8. Benzo(a)pyrene Diol Epoxide DNA Adduct Formation in Transformable and Non-Transformable Human Foreskin Fibroblast Cells in Vitro. [R] . Tejwani, R., Jeffrey, A. M., Milo, G. E. 1981

机译：苯并（a）芘二醇环氧化物DNa加合物在可转化和不可转化的人类包皮成纤维细胞体外形成。

A small nuclear RNA, U5, can transform cells in vitro.

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