Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.

T M Underhill; W F Flintoff

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Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.

机译：DNA介导的基因转移对中国仓鼠卵巢细胞中甲氨蝶呤摄取缺陷的补充。

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A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

机译：通过从野生型中国仓鼠卵巢或人G2细胞中分离的DNA转染，补充了甲氨蝶呤摄取不足的耐甲氨蝶呤的中国仓鼠卵巢细胞系，以补充甲氨蝶呤敏感性。一级和二级转染子以与野生型细胞相似的方式恢复了吸收甲氨蝶呤的能力，在那些被人DNA转染的情况下，含有人特异性DNA序列。通过这种甲氨蝶呤抗性表型的DNA介导的基因转移进行互补，为克隆涉及甲氨蝶呤摄取的基因提供了基础。

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《Molecular and Cellular Biology》 |1989年第4期|共5页
作者
T M Underhill; W F Flintoff;
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中图分类细胞生物学;
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1. Moderate-level gene amplification in methotrexate-resistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of the amplified DHFR gene. [J] . W F Flintoff, E Livingston, C Duff, Molecular and Cellular Biology . 1984,第1期

机译：在耐甲氨蝶呤的中国仓鼠卵巢细胞中中等水平的基因扩增伴随着扩增的DHFR基因位点处或附近的染色体易位。
2. Overproduction of dihydrofolate reductase and gene amplification in methotrexate-resistant Chinese hamster ovary cells. [J] . W F Flintoff, M K Weber, C R Nagainis, Molecular and Cellular Biology . 1982,第3期

机译：抗甲氨蝶呤的中国仓鼠卵巢细胞中二氢叶酸还原酶的过量生产和基因扩增。
3. DNA-mediated cotransfer of excision repair capacity and drug resistance into chinese hamster ovary mutant cell line UV-135. [J] . M A MacInnes, J M Bingham, L H Thompson, Molecular and Cellular Biology . 1984,第6期

机译：DNA介导的切除修复能力和耐药性向中国仓鼠卵巢突变细胞系UV-135的共转移。
4. Transfection System Using a Cocktail of Totally Designed Lipidic Materials in Chinese Hamster Ovary Cells: Lipidic Cocktail as Potential Gene Carrier for CHO Cells [C] . Ken-Ichi Kusumoto, Takahiro Nagata, Eiichi Kanazawal, Annual and International Meeting of the Japanese Association for Animal Cell Technology . 2010

机译：使用在中国仓鼠卵巢细胞中使用全面设计的脂质材料的鸡尾酒的转染系统：脂质鸡尾酒作为CHO细胞的潜在基因载体
5. Somatic cell and molecular genetic analysis of various Chinese hamster ovary (CHO) mutant cells defective in sterol-dependent regulation of cholesterol biosynthesis and LDL receptor expression. [D] . Hasan, Mazahir Tahir. 1993

机译：各种中国仓鼠卵巢（CHO）突变细胞的体细胞和分子遗传学分析，它们在胆固醇依赖的胆固醇生物合成和LDL受体表达调控中存在缺陷。
6. Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer. [O] . T M Underhill, W F Flintoff 1989

机译：DNA介导的基因转移对中国仓鼠卵巢细胞中甲氨蝶呤摄取缺陷的补充。
7. Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer. [O] . Underhill, T M, Flintoff, W F 1989

机译：DNA介导的基因转移对中国仓鼠卵巢细胞中甲氨蝶呤摄取缺陷的补充。
8. Results of a Chinese Hamster Ovary Cell In Vitro Chromosome Aberration Assay and a Mammalian Cell Gene-Mutation Assay in L5178Y Mouse Lymphoma Cells. [R] . 2012

机译：中国仓鼠卵巢细胞体外染色体畸变测定和哺乳动物细胞基因突变测定L5178Y小鼠淋巴瘤细胞的结果。

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.

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