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首页> 外文期刊>Molecular and Cellular Biology >Cell surface and Golgi pools of beta-1,4-galactosyltransferase are differentially regulated during embryonal carcinoma cell differentiation.
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Cell surface and Golgi pools of beta-1,4-galactosyltransferase are differentially regulated during embryonal carcinoma cell differentiation.

机译：β-1，4-半乳糖基转移酶的细胞表面和高尔基池在胚胎癌细胞分化过程中受到差异调节。

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摘要

beta-1,4-Galactosyltransferase (GalTase) has two functionally distinct subcellular distributions. In the Golgi apparatus, GalTase participates in the glycosylation of secretory and membrane-bound glycoproteins, whereas on the cell surface it mediates specific aspects of intercellular adhesion. For this study, a murine GalTase clone was obtained by screening a lambda gt10 cDNA library made from F9 embryonal carcinoma cells with a heterologous bovine GalTase cDNA probe. The murine GalTase cDNA probe was used in conjunction with assays of GalTase activity to investigate the expression and distribution of GalTase during differentiation of F9 stem cells into secretory endodermal epithelium. During the initial phase of F9 cell differentiation, GalTase mRNA levels remained relatively constant; however, as differentiation progressed, as assayed by expression of the differentiation-specific marker laminin B1, GalTase mRNA levels and enzyme activity rose dramatically. Furthermore, subcellular fractionation of these cells showed that the increased GalTase levels were specifically associated with the Golgi apparatus, whereas GalTase specific activity on the plasma membrane remained constant. These results show that levels of cell surface and Golgi GalTase change relative to one another during F9 cell differentiation and suggest that these functionally distinct pools of GalTase are independently and differentially regulated.
机译：β-1,4-半乳糖基转移酶（GalTase）具有两个功能不同的亚细胞分布。在高尔基体中，GalTase参与分泌和膜结合糖蛋白的糖基化，而在细胞表面上它介导细胞间粘附的特定方面。对于本研究，通过用异源牛GalTase cDNA探针筛选由F9胚胎癌细胞制成的gt10 cDNA文库，获得了鼠GalTase克隆。小鼠GalTase cDNA探针与GalTase活性检测结合使用，研究了F9干细胞向分泌性内皮上皮分化过程中GalTase的表达和分布。在F9细胞分化的初始阶段，GalTase mRNA水平保持相对恒定。然而，随着分化的进行，如通过分化特异性标记层粘连蛋白B1的表达所测定的，GalTase mRNA水平和酶活性急剧上升。此外，这些细胞的亚细胞分级分离显示，增加的GalTase水平与高尔基体特异性相关，而GalTase在质膜上的比活性保持恒定。这些结果表明，在F9细胞分化过程中，细胞表面和高尔基GalTase的水平相对于彼此发生变化，并表明这些功能不同的GalTase池受到独立和差异调节。

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