...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >The long terminal repeat of an endogenous intracisternal A-particle gene functions as a promoter when introduced into eucaryotic cells by transfection.
【24h】

The long terminal repeat of an endogenous intracisternal A-particle gene functions as a promoter when introduced into eucaryotic cells by transfection.

机译：当通过转染引入到真核细胞中时，内源性脑池内A粒子基因的长末端重复序列充当启动子。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe experiments designed to determine whether an endogenous intracisternal A-particle (IAP) gene randomly selected from a mouse embryo library has the potential to be transcriptionally active. Assays for IAP gene transcription were done with permanently transformed rat cells and transiently transfected monkey and mouse cells. The rat cells, which had integrated IAP gene copies, contained IAP RNA. A start site within the IAP 5' long terminal repeat (LTR) was localized by S1 mapping. The promoter activity of the IAP LTR was also measured in cells 48 h after the introduction of recombinant plasmids in which bacterial chloramphenicol acetyl transferase (CAT) encoding sequences were under the control of the LTR. The IAP LTR promoted CAT activity in mouse and monkey cells. In mouse L-cells, the levels of CAT activity were 10 to 25% of those promoted by an analogous recombinant containing the Moloney murine sarcoma virus LTR as the promoter. In contrast to the Moloney murine sarcoma virus LTR, the IAP LTR was five- to eightfold more active in monkey cells than in mouse cells. The 5' and 3' LTRs were equally active, and promoter activity was dependent on having the orientation of the LTRs with respect to the CAT gene the same as their orientation with respect to the IAP gene. A 5'-flanking sequence containing a member of the highly repetitive R-sequence family increased CAT activity in COS cells 11-fold when present along with the LTR. Our results indicate that the LTR of an endogenous mouse IAP gene can function as an efficient promoter in heterologous as well as homologous cells.
机译：我们描述了旨在确定是否从小鼠胚胎库中随机选择的内源性脑内核内A粒子（IAP）基因是否具有转录活性的潜力的实验。用永久转化的大鼠细胞以及瞬时转染的猴和小鼠细胞进行IAP基因转录的测定。具有整合IAP基因拷贝的大鼠细胞含有IAP RNA。通过S1映射对IAP 5'长末端重复序列（LTR）中的起始位点进行了定位。在引入重组质粒后48小时，还测量了IAP LTR的启动子活性，在重组质粒中，细菌氯霉素乙酰转移酶（CAT）编码序列处于LTR的控制之下。 IAP LTR促进了小鼠和猴细胞中的CAT活性。在小鼠L细胞中，CAT活性的水平是由含有莫洛尼鼠肉瘤病毒LTR作为启动子的类似重组体所促进的CAT活性的10-25％。与莫洛尼氏鼠肉瘤病毒LTR相反，IAP LTR在猴细胞中的活性是在小鼠细胞中的五到八倍。 5'和3'LTR具有相同的活性，启动子活性取决于LTR相对于CAT基因的方向与它们相对于IAP基因的方向是否相同。当含有LTR时，含有高度重复的R序列家族成员的5'侧翼序列使COS细胞中的CAT活性增加了11倍。我们的结果表明，内源性小鼠IAP基因的LTR可以在异源和同源细胞中充当有效的启动子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号