• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A consensus sequence polymer inhibits in vivo expression of heat shock genes.
【24h】

A consensus sequence polymer inhibits in vivo expression of heat shock genes.

机译：共有序列聚合物抑制热休克基因的体内表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Promoter function for hsp70 gene expression in Drosophila melanogaster was studied with an in vivo competition assay. A polymer of 40 tandem copies of the pair of regulatory elements of the hsp70 gene was constructed and cloned into a plasmid vector. Various marked genes were cotransfected with the polymer plasmid into Schneider line 2 cells, and their expression was determined by enzyme activity measurements. The polymer dramatically inhibited expression of cotransfected hsp70, hsp26, and hsp83 genes, but not cotransfected copia and histone genes. Our results indicate that in vivo, a trans-acting, positive regulatory factor, which can be titrated by heat shock consensus sequences, is required for activation of heat shock genes and is specific for these genes; the coordinate induction of different heat shock genes appears to be mediated by similar, but not identical, interactions of the trans-acting induction factor and the cis-acting heat shock consensus sequences; and the uninduced or basal level expression of the transfected hsp70 gene is also due to interaction of the consensus sequence with a positively acting factor.
机译：通过体内竞争试验研究了果蝇中hsp70基因表达的启动子功能。构建了hsp70基因的一对调控元件的40个串联拷贝的聚合物，并将其克隆到质粒载体中。将各种标记基因与聚合物质粒共转染到Schneider系2细胞中，并通过酶活性测量确定其表达。该聚合物可显着抑制共转染的hsp70，hsp26和hsp83基因的表达，但不会共转染copia和组蛋白基因。我们的结果表明，在体内，可以通过热休克共有序列滴定的反式正调控因子是激活热休克基因所必需的，并且对这些基因具有特异性。不同热休克基因的坐标诱导似乎是由反式作用诱导因子和顺式热激共有序列的相似但不相同的相互作用介导的;转染的hsp70基因的未诱导或基础表达也归因于共有序列与正性作用因子的相互作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号