Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage.

D E Helland; P W Doetsch; W A Haseltine

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Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage.

机译：哺乳动物DNA修复核酸内切酶的底物特异性，可识别氧化性碱基损伤。

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The substrate specificity of a calf thymus endonuclease on DNA damaged by UV ligh, ionizing radiation, and oxidizing agents was investigated. End-labeled DNA fragments of defined sequence were used as substrates, and the enzyme-generated scission products were analyzed by using DNA sequencing methodologies. The enzyme was shown to incise damaged DNA at pyrimidine sites. The enzyme incised DNA damaged with UV light, ionizing radiation, osmium tetroxide, potassium permanganate, and hydrogen peroxide at cytosine and thymine sites. The substrate specificity of the calf thymus endonuclease was compared to that of Escherichia coli endonuclease III. Similar pyrimidine base damage specificities were found for both enzymes. These results define a highly conserved class of enzymes present in both procaryotes and eucaryotes that may mediate an important role in the repair of oxidative DNA damage.

机译：研究了小牛胸腺核酸内切酶对被紫外线，电离辐射和氧化剂破坏的DNA的底物特异性。末端标记的确定序列的DNA片段被用作底物，并使用DNA测序方法分析了酶产生的分裂产物。该酶显示在嘧啶位点切割受损的DNA。该酶切割的DNA在胞嘧啶和胸腺嘧啶位点受到紫外线，电离辐射，四氧化，高锰酸钾和过氧化氢的破坏。将小牛胸腺核酸内切酶的底物特异性与大肠杆菌核酸内切酶III的底物特异性进行了比较。对于这两种酶发现相似的嘧啶碱基破坏特异性。这些结果确定了在原核生物和真核生物中都存在的高度保守的酶类别，它们可能在氧化性DNA损伤的修复中起重要作用。

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《Molecular and Cellular Biology》 |1986年第6期|共8页
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D E Helland; P W Doetsch; W A Haseltine;
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中图分类细胞生物学;
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1. Substrate specificities and excision kinetics of DNA glycosylases involved in base-excision repair of oxidative DNA damage. [J] . Dizdaroglu M Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 2003,第1a2期

机译：DNA糖基化酶的底物特异性和切除动力学涉及氧化性DNA损伤的碱基切除修复。
2. Mammalian DNA base excision repair proteins: their interactions and role in repair of oxidative DNA damage. [J] . Izumi T, Wiederhold LR, Roy G, Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems . 2003,第1a2期

机译：哺乳动物DNA碱基切除修复蛋白：它们的相互作用和在氧化DNA损伤修复中的作用。
3. DNA substrates containing defined oxidative base lesions and their application to study substrate specificities of base excision repair enzymes. [J] . Ide H Progress in nucleic acid research and molecular biology . 2001,第0期

机译：包含确定的氧化性碱基病变的DNA底物及其在研究碱基切除修复酶的底物特异性中的应用。
4. The mechanism of base-excision DNA repair by Endonuclease-VIII as revealed from its trapped covalent intermediate with a DNA substrate [C] . Gali Golan, Dmitry O.Zharkov, Rotem Gilboa International Peptide Symposium;European Peptide Symposium . 2005

机译：通过与DNA底物的被捕获的共价中间体揭示的内切核酸酶-VIII碱基切除DNA修复机制
5. Coordination of mammalian base excision repair of DNA by AP endonuclease 1 and DNA polymerase beta. [D] . Wong, Donny. 2003

机译：通过AP核酸内切酶1和DNA聚合酶β进行哺乳动物碱基切除修复DNA的协调。
6. Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage. [O] . D E Helland, P W Doetsch, W A Haseltine 1986

机译：哺乳动物DNA修复核酸内切酶的底物特异性可识别氧化性碱基损伤。
7. Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage. [O] . Helland, D E, Doetsch, P W, Haseltine, W A 1986

机译：哺乳动物DNA修复核酸内切酶的底物特异性，可识别氧化性碱基损伤。
8. STUDIES ON THE SUBSTRATE SPECIFICITY OF THE T4 EXCISION REPAIR ENDONUCLEASE [R] . Errol C. Friedberg 1972

机译：T4 EXCIsION修复核糖核酸酶的底物特异性研究

Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage.

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