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首页> 外文期刊>Molecular and Cellular Biology >Activation of oncogenicity of the c-rel proto-oncogene.
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Activation of oncogenicity of the c-rel proto-oncogene.

机译：c-rel原癌基因的致癌性的激活。

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摘要

Reticuloendotheliosis virus strain T (Rev-T) induces a lethal lymphoma in young birds and transforms avian lymphoid cells in vitro. The transforming gene of Rev-T, v-rel, was derived from the turkey proto-oncogene c-rel. Comparison of the nucleotide sequences of v-rel and c-rel indicates that in addition to several internal amino acid changes relative to c-rel, p59v-rel has amino acid sequences at both ends derived from the reticuloendotheliosis virus strain A-related virus env gene (K. C. Wilhelmsen, K. Eggleton, and H. M. Temin, J. Virol. 52:172-182, 1984). In this report, the v-rel sequences important for transformation were defined by constructing recombinant retroviruses in which c-rel sequences replaced the analogous v-rel sequences. These recombinant viruses expressing chimeric proteins were tested for their ability to transform spleen cells in vitro and to induce tumors in young chickens. Activation of the oncogenicity of c-rel in Rev-T required alteration of the amino terminus and the central region of the protein. Deletion of the noncoding sequences 3' to c-rel and of most of the helper virus-related env sequences was necessary for the formation of Rev-T.
机译：网状内皮炎病毒株T（Rev-T）在幼鸟中诱导致命的淋巴瘤，并在体外转化禽淋巴样细胞。 Rev-T的转化基因v-rel来自火鸡原癌基因c-rel。 v-rel和c-rel核苷酸序列的比较表明，除了相对于c-rel的一些内部氨基酸变化外，p59v-rel还在两端具有源自网状内皮病病毒株A相关病毒env的氨基酸序列基因（KC Wilhelmsen，K.Eggleton，和HM Temin，J.Virol.52：172-182，1984）。在该报告中，通过构建重组逆转录病毒定义了对于转化重要的v-rel序列，其中c-rel序列取代了类似的v-rel序列。测试了这些表达嵌合蛋白的重组病毒的体外转化脾细胞和诱导雏鸡肿瘤的能力。 Rev-T中c-rel的致癌性的激活要求氨基末端和蛋白质中心区域的改变。对于Rev-T的形成，必须删除非编码序列3'至c-rel和大多数与辅助病毒有关的env序列。

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