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首页> 外文期刊>Molecular and Cellular Biology >Rat c-raf oncogene activation by a rearrangement that produces a fused protein.
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Rat c-raf oncogene activation by a rearrangement that produces a fused protein.

机译：通过重排产生融合蛋白的大鼠c-raf癌基因激活。

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In a previous study, activated rat c-raf was detected by an NIH 3T3 cell transfection assay, and a rearrangement was demonstrated in the 5' half of the sequence of the gene. In the present study, the cDNAs of normal and activated rat c-raf were analyzed. Results showed that the activated c-raf gene is transcribed to produce a fused mRNA, in which the 5' half of the sequence is replaced by an unknown rat sequence. This mRNA codes a fused c-raf protein. The normal and activated c-raf cDNAs were each connected to the long terminal repeat of Rous sarcoma virus and transfected into NIH 3T3 cells. Only the activated form had transforming activity. We conclude that the rearrangement is responsible for the activation of c-raf.
机译：在先前的研究中，通过NIH 3T3细胞转染测定法检测到了活化的大鼠c-raf，并在该基因序列的5'半部分证实了重排。在本研究中，分析了正常和活化大鼠c-raf的cDNA。结果表明，激活的c-raf基因被转录产生融合的mRNA，其中5'的一半序列被未知的大鼠序列所取代。该mRNA编码融合的c-raf蛋白。正常和激活的c-raf cDNA均与Rous肉瘤病毒的长末端重复序列相连，并转染到NIH 3T3细胞中。仅激活形式具有转化活性。我们得出结论，重排是导致c-raf活化的原因。

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