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首页> 外文期刊>Molecular and Cellular Biology >Interaction between the yeast mitochondrial and nuclear genomes influences the abundance of novel transcripts derived from the spacer region of the nuclear ribosomal DNA repeat.
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Interaction between the yeast mitochondrial and nuclear genomes influences the abundance of novel transcripts derived from the spacer region of the nuclear ribosomal DNA repeat.

机译：酵母线粒体与核基因组之间的相互作用影响了核核糖体DNA重复序列间隔区衍生的新转录本的数量。

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摘要

We have identified stable transcripts from the so-called nontranscribed spacer region (NTS) of the nuclear ribosomal DNA repeat in certain respiration-deficient strains of Saccharomyces cerevisiae. These RNAs, which are transcribed from the same strand as is the 37S rRNA precursor, are 500 to 800 nucleotides long and extend from the 5' end of the 5S rRNA gene to three major termination sites about 1,780, 1,830, and 1,870 nucleotides from the 3' end of the 26S rRNA gene. A survey of various wild-type and respiration-deficient strains showed that NTS transcript abundance depended on the mitochondrial genotype and a single codominant nuclear locus. In strains with that nuclear determinant, NTS transcripts were barely detected in [rho+] cells, were slightly more abundant in various mit- derivatives, and were most abundant in petites. However, in one petite that was hypersuppressive and contained a putative origin of replication (ori5) within its 757-base-pair mitochondrial genome, NTS transcripts were no more abundant than in [rho+] cells. The property of low NTS transcript abundance in the hypersuppressive petite was unstable, and spontaneous segregants that contained NTS transcripts as abundant as in the other petites examined could be obtained. Thus, respiration deficiency per se is not the major factor contributing to the accumulation of these unusual RNAs. Unlike RNA polymerase I transcripts, the abundant NTS RNAs were glucose repressible, fractionated as poly(A)+ RNAs, and were sensitive to inhibition by 10 micrograms of alpha-amanitin per ml, a concentration that had no effect on rRNA synthesis. Abundant NTS RNAs are therefore most likely derived by polymerase II transcription.
机译：我们从啤酒酵母的某些呼吸不足菌株中的核糖体DNA重复序列的所谓非转录间隔区（NTS）中鉴定出稳定的转录本。这些RNA从与37S rRNA前体相同的链转录而来，长度为500至800个核苷酸，并从5S rRNA基因的5'端延伸至三个主要的终止位点，分别位于3S rRNA基因的1,780、1,830和1,870个核苷酸之间。 26S rRNA基因的3'末端。对各种野生型和缺乏呼吸的菌株进行的调查显示，NTS转录本的丰度取决于线粒体的基因型和单个显性核基因座。在具有该核决定簇的菌株中，在rho +细胞中几乎未检测到NTS转录本，其在各种mit衍生物中的含量略高，而在娇小分子中的含量最高。然而，在一个高度抑制并且在其757个碱基对的线粒体基因组中包含一个假定的复制起点（ori5）的娇小动物中，NTS转录本并不比[rho +]细胞中的丰富。高抑制性小身材中低NTS转录物丰度的特性是不稳定的，并且可以得到包含NTS转录物的自发分离物，其含量与其他小身材一样丰富。因此，呼吸不足本身并不是造成这些异常RNA积累的主要因素。与RNA聚合酶I转录本不同，大量的NTS RNA具有葡萄糖抑制性，可分离为poly（A）+ RNA，并且对每毫升10微克的α-amanitin的抑制作用敏感，该浓度对rRNA的合成没有影响。因此，大量的NTS RNA很可能是通过聚合酶II转录获得的。

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