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首页> 外文期刊>Molecular and Cellular Biology >Functional domains of the Drosophila melanogaster muscle myosin heavy-chain gene are encoded by alternatively spliced exons.
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Functional domains of the Drosophila melanogaster muscle myosin heavy-chain gene are encoded by alternatively spliced exons.

机译：果蝇黑腹肌肌球蛋白重链基因的功能域由交替剪接的外显子编码。

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The single-copy Drosophila muscle myosin heavy-chain (MHC) gene, located at 36B(2L), has a complex exon structure that produces a diversity of larval and adult muscle MHC isoforms through regulated alternative RNA splicing. Genomic and cDNA sequence analyses revealed that this 21-kilobase MHC gene encodes these MHC isoforms in 19 exons. However, five sets of these exons, encoding portions of the S1 head and the hinge domains of the MHC protein, are tandemly repeated as two, three, four, or five divergent copies, which are individually spliced into RNA transcripts. RNA hybridization studies with exon-specific probes showed that at least 10 of the 480 possible MHC isoforms that could arise by alternative RNA splicing of these exons are expressed as MHC transcripts and that the expression of specific members of alternative exon sets is regulated, both in stage and in muscle-type specificity. This regulated expression of specific exons is of particular interest because the alternatively spliced exon sets encode discrete domains of the MHC protein that likely contribute to the specialized contractile activities of different Drosophila muscle types. The alternative exon structure of the Drosophila MHC gene and the single-copy nature of this gene in the Drosophila genome make possible transgenic experiments to test the physiological functions of specific MHC protein domains and genetic and molecular experiments to investigate the mechanisms that regulate alternative exon splicing of MHC and other muscle gene transcripts.
机译：单拷贝果蝇肌肉肌球蛋白重链（MHC）基因位于36B（2L），具有复杂的外显子结构，可通过调节性RNA剪接产生各种幼虫和成年肌肉MHC同工型。基因组和cDNA序列分析表明，这个21碱基的MHC基因在19个外显子中编码这些MHC同工型。但是，这些外显子的五组，分别编码S1头部的部分和MHC蛋白的铰链结构域，以两个，三个，四个或五个不同的拷贝串联重复，分别复制成RNA转录物。用外显子特异性探针进行的RNA杂交研究表明，由这些外显子的替代RNA剪接产生的480种可能的MHC亚型中，至少有10种被表达为MHC转录本，并且替代性外显子组的特定成员的表达受到调节。阶段和肌肉类型的特异性。特定外显子的这种调节表达特别令人关注，因为交替剪接的外显子集编码MHC蛋白的离散域，这可能有助于不同果蝇肌肉类型的专门收缩活动。果蝇MHC基因的替代外显子结构以及该基因在果蝇基因组中的单拷贝性质使得转基因实验可以测试特定MHC蛋白结构域的生理功能，以及遗传和分子实验来研究调控替代外显子剪接的机制。 MHC和其他肌肉基因转录本。

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