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首页> 外文期刊>Molecular and Cellular Biology >Corrective recombination of mouse immunoglobulin kappa alleles in Abelson murine leukemia virus-transformed pre-B cells.
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Corrective recombination of mouse immunoglobulin kappa alleles in Abelson murine leukemia virus-transformed pre-B cells.

机译：小鼠免疫球蛋白κ等位基因在Abelson鼠白血病病毒转化的pre-B细胞中的正确重组。

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摘要

Previous characterization of mouse immunoglobulin kappa gene rearrangement products cloned from murine plasmacytomas has indicated that two recombination events can take place on a single kappa allele (R. M. Feddersen and B. G. Van Ness, Proc. Natl. Acad. Sci. USA 82:4792-4797, 1985; M. A. Shapiro and M. Weigert, J. Immunol. 139:3834-3839, 1987). To determine whether multiple recombinations on a single kappa allele can contribute to the formation of productive V-J genes through corrective recombinations, we have examined several Abelson murine leukemia virus-transformed pre-B-cell clones which rearrange the kappa locus during cell culture. Clonal cell lines which had rearranged one kappa allele nonproductively while maintaining the other allele in the germ line configuration were grown, and secondary subclones, which subsequently expressed kappa protein, were isolated and examined for further kappa rearrangement. A full spectrum of rearrangement patterns was observed in this sequential cloning, including productive and nonproductive recombinations of the germ line allele and secondary recombinations of the nonproductive allele. The results show that corrective V-J recombinations, with displacement of the nonproductive kappa gene, occur with a significant frequency (6 of 17 kappa-producing subclones). Both deletion and maintenance of the primary (nonfunctional) V-J join, as a reciprocal product, were observed.
机译：从鼠浆细胞瘤克隆的小鼠免疫球蛋白kappa基因重排产物的先前表征表明，在单个kappa等位基因上可发生两次重组事件（RM Feddersen和BG Van Ness，美国国家科学院院刊82：4792-4797， 1985; MA Shapiro和M.Weigert，J.Immunol.139：3834-3839，1987）。为了确定单个κ等位基因上的多个重组是否可以通过纠正性重组来促进生产性V-J基因的形成，我们检查了几种由Abelson鼠白血病病毒转化的前B细胞克隆，这些克隆在细胞培养过程中重新排列了κ基因座。生长已经非生产性地重排一个κ等位基因同时将另一等位基因维持在种系构型中的克隆细胞系，并分离随后表达κ蛋白的次级亚克隆，并检查其进一步的κ重排。在此顺序克隆中观察到了全谱的重排模式，包括种系等位基因的生产性和非生产性重组以及非生产性等位基因的次级重组。结果表明，随着非生产性κ基因的置换，纠正性V-J重组发生的频率很高（17个产生κ的亚克隆中有6个发生）。观察到主要的（非功能性）V-J连接的缺失和维持，这是相互的产物。

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