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首页> 外文期刊>Molecular and Cellular Biology >SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae.
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SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae.

机译：SNF6编码一种在酿酒酵母中表达许多基因所需的核蛋白。

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摘要

The SNF6 gene appears to affect transcription from a variety of promoters in Saccharomyces cerevisiae. The gene was cloned, and sequence analysis revealed two completely overlapping open reading frames of 996 and 1092 nucleotides on opposite strands. The SNF6 coding sequence was identified by selective mutagenesis. The predicted 37,604-dalton SNF6 protein is highly charged but overall neutral. A bifunctional SNF6-beta-galactosidase fusion protein was localized in the nucleus, as judged by immunofluorescence microscopy. The N terminus of SNF6 contains a sequence homologous to nuclear localization signals and was sufficient to direct beta-galactosidase to the nucleus. The 5' ends of the SNF6 RNA were heterogeneous and included ends mapping downstream from the first ATG codon. Construction of a frameshift mutation provided evidence that translational initiation at the second ATG yields a partially functional SNF6 product. Null mutations in SNF6 caused a wider range of pleiotropic defects than the previously isolated point mutation, including slow growth. Genetic and molecular evidence suggested that SNF6 is functionally related to the SNF2 and SNF5 genes. These genes may function together to affect transcription.
机译：SNF6基因似乎影响酿酒酵母中各种启动子的转录。克隆了该基因，并且序列分析揭示了在相对链上两个完全重叠的996和1092个核苷酸的开放阅读框。通过选择性诱变鉴定了SNF6编码序列。预测的37,604道尔顿的SNF6蛋白带电较高，但总体呈中性。通过免疫荧光显微镜检查判断，双功能SNF6-β-半乳糖苷酶融合蛋白位于细胞核中。 SNF6的N末端包含与核定位信号同源的序列，足以将β-半乳糖苷酶引导至细胞核。 SNF6 RNA的5'末端是异质的，包括位于第一个ATG密码子下游的末端。移码突变的构建提供了证据，证明第二个ATG的翻译起始产生了部分功能性SNF6产物。与以前孤立的点突变相比，SNF6中的空突变引起多效性缺陷的范围更广，包括生长缓慢。遗传和分子证据表明，SNF6与SNF2和SNF5基因在功能上相关。这些基因可能一起起作用以影响转录。

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