Comparison of the levels of the 21S mitochondrial rRNA in derepressed and glucose-repressed Saccharomyces cerevisiae.

R Kelly; S L Phillips

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Comparison of the levels of the 21S mitochondrial rRNA in derepressed and glucose-repressed Saccharomyces cerevisiae.

机译：抑制和抑制葡萄糖酿酒酵母中21S线粒体rRNA水平的比较。

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A cDNA preparation, synthesized by using Saccharomyces cerevisiae mitochondrial RNA as template and oligodeoxythymidylic acid as primer, was found to specifically hybridize to the mitochondrial 21S rRNA by the following criteria: (i) it hybridizes only to the 21S RNA species in mitochondrial RNA and not to RNA from a [rho0] mutant, and (ii) it hybridizes to fragments in restriction digests of mitochondrial DNA that contain the 21S rRNA gene but not to nuclear DNA. This cDNA was used as a probe to demonstrate that a 2.6-fold decrease in the cellular level of the mitochondrial large rRNA is associated with glucose repression of mitochondrial function in S. cerevisiae. A corresponding decrease in the level of mitochondrial DNA was not observed.

机译：发现以酿酒酵母线粒体RNA为模板，寡聚脱氧胸苷酸为引物合成的cDNA制备物，可通过以下标准与线粒体21S rRNA特异性杂交：（i）仅与线粒体RNA中的21S RNA物种杂交，而未与（ii）与含有21S rRNA基因的线粒体DNA限制性酶切片段杂交，但不与核DNA杂交。将该cDNA用作探针，以证明线粒体大rRNA的细胞水平降低2.6倍与酿酒酵母中线粒体功能的葡萄糖抑制有关。没有观察到线粒体DNA水平的相应降低。

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《Molecular and Cellular Biology》 |1983年第11期|共9页
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R Kelly; S L Phillips;
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中图分类细胞生物学;
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1. Transcriptional and translational regulation of major heat shock proteins and patterns of trehalose mobilization during hyperthermic recovery in repressed and derepressed Saccharomyces cerevisiae. [J] . Gross C, Watson K Canadian journal of microbiology . 1998,第4期

机译：在抑制和抑制的酿酒酵母中，主要热休克蛋白的转录和翻译调节以及海藻糖动员的模式在高温恢复过程中。
2. Transcriptional and translational regulation of major heat shock proteins and patterns of trehalose mobilization during hyperthermic recovery in repressed and derepressed Saccharomyces cerevisiae. [J] . Gross C, Watson K Canadian journal of microbiology . 1998,第4期

机译：在抑制和抑制的酿酒酵母中，主要热休克蛋白的转录和翻译调节以及海藻糖动员的模式在高温恢复过程中。
3. The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae. [J] . B E Morrow, S P Johnson, J R Warner Molecular and Cellular Biology . 1993,第2期

机译：rRNA增强子调节酿酒酵母中的rRNA转录。
4. Quantitative proteomic analysis reveals environmental interaction and epistasis in the responses to complex stimuli in Saccharomyces cerevisiae. [C] . Parimal Samir, Rahul, James C. Slaughter, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：定量蛋白质组学分析揭示了对酿酒酵母酿酒酵母复杂刺激的反应中的环境相互作用和外观。
5. Characterization of components required to maintain mitochondrial DNA and mitochondrial morphology in the yeast, Saccharomyces cerevisiae. [D] . Wong, Edith Denise. 2002

机译：维持酵母酵母中线粒体DNA和线粒体形态所需的成分的表征。
6. Comparison of the levels of the 21S mitochondrial rRNA in derepressed and glucose-repressed Saccharomyces cerevisiae. [O] . R Kelly, S L Phillips 1983

机译：抑制和抑制葡萄糖酿酒酵母中21S线粒体rRNA水平的比较。
7. Comparison of the levels of the 21S mitochondrial rRNA in derepressed and glucose-repressed Saccharomyces cerevisiae. [O] . Kelly, R, Phillips, S L 1983

机译：抑制和抑制葡萄糖酿酒酵母中21S线粒体rRNA水平的比较。

Comparison of the levels of the 21S mitochondrial rRNA in derepressed and glucose-repressed Saccharomyces cerevisiae.

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