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首页> 外文期刊>Molecular and Cellular Biology >Synthesis of predominantly unspliced cytoplasmic RNAs by chimeric herpes simplex virus type 1 thymidine kinase-human beta-globin genes.
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Synthesis of predominantly unspliced cytoplasmic RNAs by chimeric herpes simplex virus type 1 thymidine kinase-human beta-globin genes.

机译：嵌合的单纯疱疹病毒1型胸苷激酶-人β-珠蛋白基因合成的主要未剪接的胞质RNA。

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摘要

The herpes simplex virus type 1 thymidine kinase (tk) gene lacks introns and produces stable mRNA in the absence of splicing. We have prepared a hybrid gene by placing the first exon, first intron (first intervening sequence, designated IVS1), and most of the second exon of the normal human beta-globin gene into the 3' untranslated region of the tk gene. Although this hybrid gene contains all globin sequences presumed necessary for the splicing of IVS1, predominantly, unspliced stable cytoplasmic RNA is produced in both long- and short-term expression assays. Moreover, stable unspliced cytoplasmic RNA is detected whether the intron is situated in a sense or an antisense orientation. Efficient splicing of IVS1 is obtained either by deleting the majority of tk coding sequences or by relocating the globin sequences from the 3' to the 5' untranslated region of the tk gene.
机译：单纯疱疹病毒1型胸苷激酶（tk）基因缺少内含子，在没有剪接的情况下产生稳定的mRNA。我们通过将正常人β-珠蛋白基因的第一个外显子，第一个内含子（第一个插入序列，指定为IVS1）和大部分第二个外显子放入tk基因的3'非翻译区中来制备杂种基因。尽管此杂种基因包含所有IVS1拼接所需的球蛋白序列，但在长期和短期表达试验中，仍会产生未拼接的稳定细胞质RNA。此外，无论内含子是有义还是反义方向，都可以检测到稳定的未剪接的胞质RNA。通过缺失大部分tk编码序列或通过将球蛋白序列从tk基因的3'非翻译区重新定位到5'可获得IVS1的有效剪接。

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