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首页> 外文期刊>Molecular and Cellular Biology >Role of specific simian virus 40 sequences in the nuclease-sensitive structure in viral chromatin.
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Role of specific simian virus 40 sequences in the nuclease-sensitive structure in viral chromatin.

机译：特定猿猴病毒40序列在病毒染色质中核酸酶敏感结构中的作用。

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摘要

A nuclease-sensitive region forms in chromatin containing a 273-base-pair (bp) segment of simian virus 40 DNA encompassing the viral origin of replication and early and late promoters. We have saturated this region with short deletion mutations and compared the nuclease sensitivity of each mutated segment to that of an unaltered segment elsewhere in the partially duplicated mutant. Although no single DNA segment is required for the formation of a nuclease-sensitive region, a deletion mutation (dl45) which disrupted both exact copies of the 21-bp repeats substantially reduced nuclease sensitivity. Deletion mutations limited to only one copy of the 21-bp repeats had little, if any, effect. A mutant (dl135) lacking all copies of the 21- and 72-bp repeats, while retaining the origin of replication and the TATA box, did not exhibit a nuclease-sensitive region. Mutants which showed reduced nuclease sensitivity had this effect throughout the nuclease-sensitive region, not just at the site of the deletion, indicating that although multiple determinants must be responsible for the nuclease-sensitive chromatin structure they do not function with complete independence. Mutant dl9, which lacks the late portion of the 72-bp segment, showed reduced accessibility to BglI, even though the BglI site is 146 bp away from the site of the deletion.
机译：染色质中形成一个核酸酶敏感区，其中包含猿猴病毒40 DNA的273个碱基对（bp）片段，该片段包含病毒的复制起点和早期和晚期启动子。我们已经用短缺失突变使该区域饱和，并比较了部分突变的突变体中每个突变区段与未改变区段的核酸酶敏感性。尽管不需要单个DNA片段来形成核酸酶敏感区，但是破坏21bp的两个精确拷贝的缺失突变（dl45）重复实质上降低了核酸酶敏感性。缺失突变仅限于21 bp重复的一个拷贝，几乎没有影响。缺少所有21-和72-bp重复拷贝的突变体（dl135），虽然保留了复制起点和TATA盒，但未显示核酸酶敏感区。显示出降低的核酸酶敏感性的突变体在整个核酸酶敏感区域具有这种作用，而不仅仅是在缺失位点，这表明尽管多个决定簇必须对核酸酶敏感的染色质结构负责，但它们并不是完全独立地起作用。缺少72bp区段的晚期部分的突变体dl9显示出对BglI的可及性降低，即使BglI位点距离缺失位点146bp。

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