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首页> 外文期刊>Molecular and Cellular Biology >Precise assignment of the light-strand promoter of mouse mitochondrial DNA: a functional promoter consists of multiple upstream domains.
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Precise assignment of the light-strand promoter of mouse mitochondrial DNA: a functional promoter consists of multiple upstream domains.

机译：小鼠线粒体DNA轻链启动子的精确分配：功能性启动子由多个上游域组成。

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摘要

Using deletion mutagenesis we localized the promoter for the light strand of mouse mitochondrial DNA to a 97-base-pair region, from -88 to +9 nucleotides of the transcriptional initiation site. Within this region the light-strand promoter could be dissected into at least three different functional domains. The specificity region, a maximum of 19 base pairs between -10 and +9 of the transcriptional initiation site, was essential and sufficient for accurate transcriptional initiation. A second region, extending to -29 nucleotides from the initiation site, facilitated the formation of a preinitiation complex between the template DNA and factor(s) present in the mitochondrial RNA polymerase fraction and was required for efficient transcription. A third, ill-defined upstream region, which extended up to -88 nucleotides from the initiation site, appeared to influence template transcriptional efficiencies in competition assays. Without the specificity domain, the upstream regions were incapable of supporting any transcription. The presence of multiple upstream domains was confirmed by disrupting nucleotide sequences in the upstream region by using linker insertion and linker replacement techniques.
机译：使用缺失诱变，我们将小鼠线粒体DNA轻链的启动子定位于一个97个碱基对的区域，从转录起始位点的-88到+9个核苷酸。在该区域内，光链启动子可以被分成至少三个不同的功能域。特异性区域，在转录起始位点的-10和+9之间最多有19个碱基对，对于准确的转录起始是必不可少的。从起始位点延伸至-29个核苷酸的第二个区域促进模板DNA和线粒体RNA聚合酶级分中存在的因子之间的预起始复合物的形成，并且是有效转录所必需的。第三个不确定的上游区域从起始位点延伸至-88个核苷酸，似乎影响了竞争测定中的模板转录效率。没有特异性结构域，上游区域不能支持任何转录。通过使用接头插入和接头替换技术破坏上游区域中的核苷酸序列，证实了多个上游结构域的存在。

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