Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.

J A Lewis; D A Matkovich

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Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.

机译：胸腺嘧啶激酶mRNA序列中包含中国仓鼠胸苷激酶基因的生长阶段依赖性和腺病毒5应答表达的遗传决定因素。

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We have constructed a chimeric thymidine kinase (TK) minigene, pHe delta 6Ha, which combines the complete coding and 3' noncoding regions of a Chinese hamster TK cDNA with the promoter region and 5' untranslated region of the TK gene of herpes simplex virus type 1. We have transformed rat 4 cells to Tk+ with this gene and analyzed the pattern of TK gene expression in these transformants under various conditions of in vitro cell culture. We find that TK gene expression in these Tk+ transformants is growth phase dependent, responsive to adenovirus 5 infection, and indistinguishable in character under a variety of cell culture conditions from the pattern of TK gene expression in rat 4 cells transformed to Tk+ with the genomic Chinese hamster TK gene clone lambda HaTK.5. We are led to the conclusion that the genetic elements which mediate growth phase-dependent TK gene expression are contained entirely within the sequences of the mature cytoplasmic hamster TK mRNA.

机译：我们构建了一个嵌合胸苷激酶（TK）小基因，pHe delta 6Ha，该基因将中国仓鼠TK cDNA的完整编码区和3'非编码区与单纯疱疹病毒类型的TK基因的启动子区和5'非翻译区结合在一起1.我们已使用该基因将大鼠4细胞转化为Tk +，并分析了在各种体外细胞培养条件下这些转化子中TK基因表达的模式。我们发现这些Tk +转化子中的TK基因表达是生长阶段依赖性的，对腺病毒5感染有反应，并且在多种细胞培养条件下，其特性与从基因组中国转化为Tk +的大鼠4细胞中TK基因表达的模式没有区别。仓鼠TK基因克隆λHaTK.5。我们得出的结论是，介导生长阶段依赖性TK基因表达的遗传元件完全包含在成熟细胞质仓鼠TK mRNA的序列内。

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《Molecular and Cellular Biology》 |1986年第6期|共5页
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J A Lewis; D A Matkovich;
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中图分类细胞生物学;
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6. Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences. [O] . J A Lewis, D A Matkovich 1986

机译：中国仓鼠胸苷激酶基因的生长阶段依赖性和腺病毒5响应表达的遗传决定因素被包含在胸苷激酶mRNA序列中。
7. Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences. [O] . Lewis, J A, Matkovich, D A 1986

机译：中国仓鼠胸苷激酶基因的生长阶段依赖性和腺病毒5响应表达的遗传决定因素被包含在胸苷激酶mRNA序列中。
8. Two Final Draft Reports of an in vitro Mammalian Cell Gene Mutation Assay at the Thymidine Kinase locus TK(plus or minus) and an in vitro Chromosome Aberration Test in Chinese Hamster Ovary (CHO-WBL) Cells Using 4-Cyclohexene-1,2-dicarboxylic Acid, 2,2-di [R] . 2013

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Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.

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