Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.

J A Baum; R Geever; N H Giles

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Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.

机译：qa-1F激活蛋白的表达：qa基因簇中上游结合位点的鉴定和DNA结合域的定位。

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The qa-1F regulatory gene of Neurospora crassa encodes an activator protein required for quinic acid induction of transcription in the qa gene cluster. This activator protein was expressed in insect cell culture with a baculovirus expression vector. The activator binds to 13 sites in the gene cluster that are characterized by a conserved 16-base-pair sequence of partial dyad symmetry. One site is located between the divergently transcribed qa-1F and qa-1S regulatory genes, corroborating prior evidence that qa-1F is autoregulated and controls expression of the qa-1S repressor. Multiple upstream sites located at variable positions 5' to the qa structural genes appear to allow for greater transcriptional control by qa-1F. Full-length and truncated activator peptides were synthesized in vitro, and the DNA-binding domain was localized to the first 183 amino acids. A 28-amino acid sequence within this region shows striking homology to N-terminal sequences from other lower-eucaryotic activator proteins. A qa-1F(Ts) mutation is located within this putative DNA-binding domain.

机译：Neurospora crassa的qa-1F调控基因编码奎宁酸诱导qa基因簇中转录所需的激活蛋白。该激活蛋白用杆状病毒表达载体在昆虫细胞培养物中表达。激活物与基因簇中的13个位点结合，这些位点以保守的16部分对偶对称的16个碱基对序列为特征。一个位点位于不同转录的qa-1F和qa-1S调控基因之间，从而证实了qa-1F被自动调节并控制qa-1S阻遏物表达的先前证据。位于qa结构基因5'可变位置的多个上游位点似乎允许qa-1F更好地控制转录。全长和截短的激活肽在体外合成，并且DNA结合域定位于前183个氨基酸。该区域内的28个氨基酸序列与其他低等真核生物激活蛋白的N端序列具有惊人的同源性。 qa-1F（Ts）突变位于该推定的DNA结合域内。

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《Molecular and Cellular Biology》 |1987年第3期|共11页
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J A Baum; R Geever; N H Giles;
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中图分类细胞生物学;
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6. Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain. [O] . J A Baum, R Geever, N H Giles 1987

机译：qa-1F激活蛋白的表达：qa基因簇中上游结合位点的鉴定和DNA结合域的定位。
7. Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain. [O] . Baum, J A, Geever, R, Giles, N H 1987

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Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.

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