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首页> 外文期刊>Molecular and Cellular Biology >The two gene pairs encoding H2A and H2B play different roles in the Saccharomyces cerevisiae life cycle.
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The two gene pairs encoding H2A and H2B play different roles in the Saccharomyces cerevisiae life cycle.

机译:编码H2A和H2B的两个基因对在酿酒酵母的生命周期中发挥不同的作用。

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We have isolated Saccharomyces cerevisiae mutants bearing deletions of one or the other of the two divergently transcribed gene pairs encoding H2A and H2B. The deletions produced diverse effects on the yeast life cycle. Deletion of TRT1, one of the H2A-H2B gene pair sets, affected mitotic growth, sporulation, spore germination, the heat shock response, and exit from the stationary phase; deletion of TRT2, the other H2A-H2B gene pair set, had negligible effects on these same processes. Using a genetic complementation assay, we found that the differential effects of the deletions could be attributed to two features of the gene sets: first, the expression of the TRT1 gene pair, but not the TRT2 gene pair, could compensate for the absence of its partner; second, the protein subtypes encoded by the two gene pairs appear to have different functions in the heat shock response.
机译:我们已经分离出带有两个编码H2A和H2B的两个不同转录的基因对中的一个或另一个缺失的酿酒酵母突变体。缺失对酵母的生命周期产生了多种影响。 H2A-H2B基因对之一TRT1的缺失影响有丝分裂的生长,孢子形成,孢子萌发,热休克反应以及从固定相退出;其他H2A-H2B基因对集TRT2的缺失对这些相同过程的影响可忽略不计。使用遗传互补测定法,我们发现缺失的差异作用可以归因于基因组的两个特征:首先,TRT1基因对而非TRT2基因对的表达可以弥补其缺失。伙伴;其次,由两个基因对编码的蛋白质亚型在热激反应中似乎具有不同的功能。

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