Regulated expression of a complete human beta-globin gene encoded by a transmissible retrovirus vector.

R D Cone; A Weber-Benarous; D Baorto; R C Mulligan

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Regulated expression of a complete human beta-globin gene encoded by a transmissible retrovirus vector.

机译：由可逆转录病毒载体编码的完整人β-珠蛋白基因的调控表达。

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We introduced a human beta-globin gene into murine erythroleukemia (MEL) cells by infection with recombinant retroviruses containing the complete genomic globin sequence. The beta-globin gene was correctly regulated during differentiation, steady-state mRNA levels being induced 5- to 30-fold after treatment of the cells with the chemical inducer dimethyl sulfoxide. Studies using vectors which yield integrated proviruses lacking transcriptional enhancer sequences indicated that neither retroviral transcription nor the retroviral enhancer sequences themselves had any obvious effect on expression of the globin gene. Viral RNA expression also appeared inducible, being considerably depressed in uninduced MEL cells but approaching normal wild-type levels after dimethyl sulfoxide treatment. We provide data which suggest that the control point for both repression and subsequent activation of virus expression in MEL cells lies in the viral enhancer element.

机译：我们通过包含完整基因组球蛋白序列的重组逆转录病毒感染，将人β-球蛋白基因引入鼠类红细胞白血病（MEL）细胞。 β-珠蛋白基因在分化过程中得到了正确调节，用化学诱导剂二甲基亚砜处理细胞后，稳态mRNA水平被诱导了5至30倍。使用产生缺乏转录增强子序列的整合的原病毒的载体的研究表明，逆转录病毒转录或逆转录病毒增强子序列本身都不会对球蛋白基因的表达产生任何明显的影响。病毒RNA的表达也似乎是可诱导的，在未诱导的MEL细胞中明显降低，但在二甲基亚砜处理后达到正常的野生型水平。我们提供的数据表明，抑制和随后激活MEL细胞中病毒表达的控制点在于病毒增强子。

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《Molecular and Cellular Biology》 |1987年第2期|共11页
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R D Cone; A Weber-Benarous; D Baorto; R C Mulligan;
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中图分类细胞生物学;
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2. A majority of mice show long-term expression of a human beta-globin gene after retrovirus transfer into hematopoietic stem cells. [J] . M A Bender, R E Gelinas, A D Miller Molecular and Cellular Biology . 1989,第4期

机译：在逆转录病毒转移到造血干细胞中后，大多数小鼠表现出人类β-珠蛋白基因的长期表达。
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5. REGULATION OF CHICKEN BETA-GLOBIN GENE EXPRESSION (RETROVIRUS VECTOR, AVIAN ERYTHROBLASTOSIS VIRUS). [D] . CHOI, OK-RYUN BAIK. 1986

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机译：传染性逆转录病毒载体编码的完整人β-珠蛋白基因的调控表达。
7. Regulated expression of a complete human beta-globin gene encoded by a transmissible retrovirus vector. [O] . Cone, R D, Weber-Benarous, A, Baorto, D, 1987

机译：传染性逆转录病毒载体编码的完整人β-珠蛋白基因的调控表达。
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Regulated expression of a complete human beta-globin gene encoded by a transmissible retrovirus vector.

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