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首页> 外文期刊>Molecular and Cellular Biology >B-cell control region at the 5' end of a major histocompatibility complex class II gene: sequences and factors.
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B-cell control region at the 5' end of a major histocompatibility complex class II gene: sequences and factors.

机译：主要组织相容性复合体II类基因的5'端的B细胞控制区：序列和因子。

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Transcription of major histocompatibility complex class II genes is elaborately regulated. Mouse class II genes are transcribed primarily in B cells, peripheral macrophages and interdigitating cells, and thymic cortical and medullary cells. In this study, we began to identify the DNA sequences and protein factors that control expression of a class II gene in B cells, addressing in particular how closely they resemble those that regulate immunoglobulin gene expression. We describe a region upstream of the E alpha gene that is crucial for its transcription in the B cells of transgenic mice but is less important in cultured B-cell lines. The sequence of this region reveals several familiar motifs, including a second X-Y pair reminiscent of that residing in the promoter-proximal region of all class II genes, a B motif strikingly homologous to that associated with the immunoglobulin kappa gene enhancer, several Ephrussi motifs, and a Pu box-like sequence very similar to that implicated in simian virus 40 and lymphotrophic papovavirus expression in B cells. Careful study of the proteins that bind specifically to these different motifs prompts us to suggest that major histocompatibility complex class II and immunoglobulin genes rely on quite different factors to achieve B-cell-specific expression.
机译：主要组织相容性复杂的II类基因的转录受到严格调控。小鼠II类基因主要在B细胞，外周巨噬细胞和指间细胞以及胸腺皮质和髓样细胞中转录。在这项研究中，我们开始鉴定控制B细胞中II类基因表达的DNA序列和蛋白质因子，特别是研究它们与调节免疫球蛋白基因表达的相似程度。我们描述了E alpha基因上游的区域，该区域对其在转基因小鼠B细胞中的转录至关重要，但在培养的B细胞系中不那么重要。该区域的序列揭示了几个熟悉的基序，包括第二个XY对，使它们让人联想到所有II类基因的启动子附近区域;一个B基序与免疫球蛋白kappa基因增强子显着同源;几个Ephrussi基序，和类似于框框猿猴病毒40和B细胞中的淋巴营养性乳头状病毒表达的Pu盒样序列非常相似。对与这些不同基序特异性结合的蛋白质的仔细研究促使我们提出，主要的组织相容性复合物II类和免疫球蛋白基因依赖于完全不同的因素来实现B细胞特异性表达。

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