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首页> 外文期刊>Molecular and Cellular Biology >Tumor promoter and epidermal growth factor stimulate phosphorylation of the c-erbB-2 gene product in MKN-7 human adenocarcinoma cells.
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Tumor promoter and epidermal growth factor stimulate phosphorylation of the c-erbB-2 gene product in MKN-7 human adenocarcinoma cells.

机译：肿瘤启动子和表皮生长因子刺激MKN-7人腺癌细胞中c-erbB-2基因产物的磷酸化。

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摘要

Treatment of human adenocarcinoma MKN-7 cells with epidermal growth factor (EGF) or phorbol tetradecanoate acetate (TPA) stimulated phosphorylation of the c-erbB-2 gene product. EGF induced a rapid increase in phosphotyrosine followed by relatively gradual increases in phosphoserine and phosphothreonine. On the other hand, the TPA-induced increase in phosphorylations occurred exclusively on serine and threonine residues. Tryptic phosphopeptide mapping analysis suggested that treatments with EGF and TPA induced phosphorylation of many common sites in the c-erbB-2 gene product. However, in contrast to TPA, EGF increased the phosphorylation of the c-erbB-2 protein in cells whose protein kinase C had been down-regulated by long-term pretreatment with TPA, suggesting that EGF and TPA induce phosphorylation by different mechanisms. Since the c-erbB-2 gene product did not show detectable EGF-binding activity, phosphorylation of tyrosine of the c-erbB-2 gene product might be catalyzed directly by the EGF receptor kinase that was activated by EGF.
机译：用表皮生长因子（EGF）或佛波十四酸乙酸酯（TPA）刺激c-erbB-2基因产物的磷酸化治疗人腺癌MKN-7细胞。 EGF诱导磷酸酪氨酸迅速增加，随后磷酸丝氨酸和磷酸苏氨酸相对逐渐增加。另一方面，TPA诱导的磷酸化增加仅发生在丝氨酸和苏氨酸残基上。胰蛋白酶磷酸肽图分析表明，用EGF和TPA处理可诱导c-erbB-2基因产物中许多常见位点的磷酸化。但是，与TPA相比，EGF增加了蛋白激酶C已被TPA长期预处理下调的细胞中c-erbB-2蛋白的磷酸化，表明EGF和TPA通过不同的机制诱导了磷酸化。由于c-erbB-2基因产物没有显示出可检测的EGF结合活性，因此c-erbB-2基因产物的酪氨酸磷酸化可能直接被EGF激活的EGF受体激酶催化。

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