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首页> 外文期刊>Molecular and Cellular Biology >The COUP transcription factor binds to an upstream promoter element of the rat insulin II gene.
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The COUP transcription factor binds to an upstream promoter element of the rat insulin II gene.

机译：COUP转录因子与大鼠胰岛素II基因的上游启动子元件结合。

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摘要

Band-shifting and DNase I-footprinting assays have been used to study the trans-acting factor(s) binding to an important promoter element (-53 to -46 relative to the transcription start) of the rat insulin II gene. A binding activity which footprints a region between -60 and -40 was found in both HIT, a hamster insulinoma cell line, and HeLa cells. A mutation within this region which drastically decreases promoter activity in vivo also greatly reduces binding activity in vitro. This binding activity was purified from HeLa cells and identified by competition and renaturation analyses as being the same as the COUP (chicken ovalbumin upstream promoter) transcription factor, a DNA-binding protein required for efficient transcription of the ovalbumin gene in vitro. Interestingly, the binding sequences of the COUP transcription factor in the ovalbumin and the insulin promoters have only limited similarities.
机译：带移和DNase I足迹测定法已用于研究与大鼠胰岛素II基因的重要启动子元件（相对于转录起点为-53至-46）结合的反式作用因子。在HIT，仓鼠胰岛素瘤细胞系和HeLa细胞中都发现了一个结合活性，该结合活性覆盖了-60和-40之间的区域。该区域内的突变会大大降低体内启动子的活性，也大大降低了体外的结合活性。从HeLa细胞中纯化出这种结合活性，并通过竞争和复性分析将其鉴定为与COUP（鸡卵清蛋白上游启动子）转录因子相同，后者是在体外有效卵清蛋白基因有效转录所需的DNA结合蛋白。有趣的是，卵清蛋白和胰岛素启动子中的COUP转录因子的结合序列仅具有有限的相似性。

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