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首页> 外文期刊>Molecular and Cellular Biology >A Swiss 3T3 variant cell line resistant to the effects of tumor promoters cannot be transformed by src.
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A Swiss 3T3 variant cell line resistant to the effects of tumor promoters cannot be transformed by src.

机译：src无法转化对肿瘤启动子具有抗性的Swiss 3T3变异细胞系。

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To study the relationship between oncogenesis by v-src and normal cellular signalling pathways, we determined the effects of v-src on 3T3-TNR9 cells, a Swiss 3T3 variant which does not respond mitogenically to tumor promoters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We found that src was unable to transform these variant cells, whether the oncogene was introduced by infection with a murine retrovirus vector or by transfection with plasmid DNA. 3T3-TNR9 cells were not inherently resistant to transformation, since infection with similar recombinant retroviruses containing either v-ras or v-abl did induce transformation. Further analysis of Swiss 3T3 and 3T3-TNR9 cell populations infected with the v-src-containing retrovirus revealed that although the amount of v-src DNA in each was approximately the same, the level of the v-src message and protein and the overall level of phosphotyrosine expressed in the infected variants was much less than in infected parental cells. Cotransfection experiments using separate v-src and neo plasmids revealed a decrease in the number of G418-resistant colonies when transfections of TNR9 cells occurred in the presence of the src-containing plasmid, suggesting a growth inhibitory effect of v-src on 3T3-TNR9 cells, as has also been found for TPA itself. Since v-src cannot transform this variant cell line, which does not respond mitogenically to the protein kinase C agonist TPA, we suggest that src makes use of the protein kinase C pathway as part of its signalling activities.
机译：为了研究v-src致癌与正常细胞信号通路之间的关系，我们确定了v-src对3T3-TNR9细胞的影响，3T3-TNR9细胞是一种瑞士3T3变体，对肿瘤启动子（如12-O-十四烷酰-佛波13乙酸酯（TPA）。我们发现无论是通过鼠逆转录病毒载体感染还是质粒DNA转染引入癌基因，src都无法转化这些变异细胞。 3T3-TNR9细胞并非固有地对转化具有抗性，因为用含有v-ras或v-abl的相似重组逆转录病毒感染确实会诱导转化。对受含v-src逆转录病毒感染的Swiss 3T3和3T3-TNR9细胞群体的进一步分析表明，尽管每种病毒中v-src DNA的量大致相同，但v-src信息和蛋白质的水平以及总体在感染的变异体中表达的磷酸酪氨酸水平要比在感染的亲代细胞中低得多。使用单独的v-src和neo质粒的共转染实验表明，当在含src的质粒存在下进行TNR9细胞转染时，G418耐药菌落的数量减少，这表明v-src对3T3-TNR9的生长具有抑制作用TPA本身也发现了这种细胞。由于v-src无法转化此变体细胞系，该变体细胞系不会对蛋白激酶C激动剂TPA进行有丝分裂反应，因此我们建议src利用蛋白激酶C途径作为其信号传导活性的一部分。

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