...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Lipopolysaccharide and dexamethasone induce mouse mammary tumor proviral gene expression and differentiation in B lymphocytes through distinct regulatory pathways.
【24h】

Lipopolysaccharide and dexamethasone induce mouse mammary tumor proviral gene expression and differentiation in B lymphocytes through distinct regulatory pathways.

机译：脂多糖和地塞米松通过不同的调控途径诱导小鼠乳腺肿瘤原病毒基因在B淋巴细胞中的表达和分化。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Endogenous mouse mammary tumor virus (MMTV) proviral transcripts are up regulated during the normal course of B-lymphocyte differentiation. We report here that the regulatory mechanisms which lead to increased levels of MMTV transcripts in differentiating, lipopolysaccharide (LPS)-stimulated normal B cells and in the inducible B-cell lymphoma line CH12 are at least partially distinct from those controlling increases in immunoglobulin and J-chain gene expression. In studies designed to characterize the stimulatory pathways leading to MMTV expression in CH12 cells, we found that stimulation with either LPS or dexamethasone (Dex), a transcriptional activator of MMTV genes, induced not only MMTV expression but also differentiation to antibody secretion. Only Dex-induced and not LPS-induced MMTV expression and differentiation were inhibited by the glucocorticoid antagonist RU486, demonstrating that Dex and LPS stimulate B cells by distinct molecular pathways. Therefore, in B cells, MMTV expression can be regulated via either the conventional hormone receptor-dependent pathway or a hormone receptor-independent pathway. Furthermore, these results suggest that steroid stimulation of B cells can lead to alterations in the expression of other results suggest that steroid stimulation of B cells can lead to alterations in the expression of other steroid-responsive genes that can become involved in the process of B-cell differentiation.
机译：内源性小鼠乳腺肿瘤病毒（MMTV）原病毒转录本在B淋巴细胞分化的正常过程中被上调。我们在此报告，导致分化，脂多糖（LPS）刺激的正常B细胞​​和诱导型B细胞淋巴瘤细胞系CH12的MMTV转录水平提高的调节机制至少部分不同于那些控制免疫球蛋白和J升高的调节机制链基因表达。在旨在表征导致CH12细胞中MMTV表达的刺激途径的研究中，我们发现LPS或地塞米松（Dex）（MMTV基因的转录激活剂）刺激不仅诱导MMTV表达，而且诱导抗体分泌分化。糖皮质激素拮抗剂RU486只能抑制Dex诱导的而非LPS诱导的MMTV的表达和分化，这表明Dex和LPS通过不同的分子途径刺激B细胞。因此，在B细胞中，MMTV表达可以通过常规激素受体依赖性途径或激素受体非依赖性途径来调节。此外，这些结果表明类固醇刺激B细胞可能导致其他表达的改变。这表明类固醇刺激B细胞可能导致其他可能参与B进程的类固醇反应性基因的表达改变。细胞分化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号