• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Retinoic acid increases zif268 early gene expression in rat preosteoblastic cells.
【24h】

Retinoic acid increases zif268 early gene expression in rat preosteoblastic cells.

机译：维甲酸可增加大鼠成骨前成骨细胞中zif268早期基因表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study we demonstrate that retinoic acid (RA) increases the expression of transcription factor zif268 mRNA in primary cultures of fetal rat calvarial cells and in simian virus 40-immortalized clonal rat calvarial preosteoblastic cells (RCT-1), which differentiate in response to RA, but not in the more differentiated RCT-3 and ROS 17/2.8 cells. The increased expression of zif268 mRNA is rapid (maximal within 1 h), transient (returns to basal levels by 3 h), detectable at RA doses of 10(-12)M, and independent of protein synthesis. The relative stimulation of zif268 mRNA by RA was much larger than that of other early genes, including c-fos, c-jun, and junB. The rate of transcription of RA-stimulated RCT-1 cells, estimated by nuclear run-on assays, was elevated, suggesting that RA regulation of zif268 gene transcription was at least in part transcriptional. Moreover, RA stimulated the transcriptional activity of a Zif268CAT (chloramphenicol acetyltransferase) plasmid containing 632 bp of zif268 5' regulatory sequences in RCT-1 cells but not in the more differentiated RCT-3 cells. These in vitro data support the in vivo observations which localize zif268 and RA receptor-gamma transcripts to bone and cartilage during development, suggesting that both RA and zif268 may play a role in osteoblast differentiation.
机译：在这项研究中，我们证明了视黄酸（RA）增加了胎鼠颅盖细胞的原代培养物中和猿猴病毒40永生化克隆大鼠颅盖前成骨细胞（RCT-1）中的转录因子zif268 mRNA的表达，这些细胞在响应RA，但未分化更强的RCT-3和ROS 17 / 2.8细胞。 zif268 mRNA的表达增加是快速的（在1 h内最大），瞬时的（在3 h内恢复到基础水平），在RA剂量为10（-12）M时可检测到，并且与蛋白质合成无关。 RA对zif268 mRNA的相对刺激作用远大于其他早期基因，包括c-fos，c-jun和junB。通过核运行分析估计，RA刺激的RCT-1细胞的转录速率提高了，这表明RA对zif268基因转录的调控至少是部分转录的。此外，RA在RCT-1细胞中刺激了包含632 bp zif268 5'调控序列的Zif268CAT（氯霉素乙酰转移酶）质粒的转录活性，而在更高分化的RCT-3细胞中则没有。这些体外数据支持体内观察，这些观察将zif268和RA受体-γ转录物在发育过程中定位在骨骼和软骨上，表明RA和zif268都可能在成骨细胞分化中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号