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首页> 外文期刊>Molecular and Cellular Biology >Nonhomologous recombination at sites within the mouse JH-C delta locus accompanies C mu deletion and switch to immunoglobulin D secretion.
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Nonhomologous recombination at sites within the mouse JH-C delta locus accompanies C mu deletion and switch to immunoglobulin D secretion.

机译：小鼠JH-Cδ基因座内的位点的非同源重组伴随着C mu缺失并转换为免疫球蛋白D分泌。

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摘要

Plasma cells secrete immunoglobulins other than immunoglobulin M (IgM) after a deletion and recombination in which a portion of the immunoglobulin heavy-chain locus (IgH), from the 5'-flanking region of the mu constant-region gene (C mu) to the 5'-flanking region of the secreted heavy-chain constant-region gene (CH), is deleted. The recombination step is believed to be targeted via switch regions, stretches of repetitive DNA which lie in the 5' flank of all CH genes except delta. Although serum levels of IgD are very low, particularly in the mouse, IgD-secreting plasmacytomas of BALB/c and C57BL/6 mice are known. In an earlier study of two BALB/c IgD-secreting hybridomas, we reported that both had deleted the C mu gene, and we concluded that this deletion was common in the normal generation of IgD-secreting cells. To learn how such switch recombinations occur in the absence of a switch region upstream of the C delta 1 exon, we isolated seven more BALB/c and two C57BL/6 IgD-secreting hybridomas. We determined the DNA sequences of the switch recombination junctions in eight of these hybridomas as well as that of the C57BL/6 hybridoma B1-8. delta 1 and of the BALB/c, IgD-secreting plasmacytoma TEPC 1033. All of the lines had deleted the C mu gene, and three had deleted the C delta 1 exon in the switch recombination event. The delta switch recombination junction sequences were similar to those of published productive switch recombinations occurring 5' to other heavy-chain genes, suggesting that nonhomologous, illegitimate recombination is utilized whenever the heavy-chain switch region is involved in recombination.
机译：血浆细胞从mu恒定区基因（C mu）的5'侧翼区缺失到重组后，分泌出除免疫球蛋白M（IgM）以外的免疫球蛋白，其中一部分免疫球蛋白重链基因座（IgH）分泌的重链恒定区基因（CH）的5'侧翼区被删除。据信重组步骤是通过开关区域，位于除了CH3以外的所有CH基因的5'侧翼的重复DNA片段来靶向的。尽管IgD的血清水平非常低，尤其是在小鼠中，但已知BALB / c和C57BL / 6小鼠分泌IgD的浆细胞瘤。在对两个BALB / c分泌IgD的杂交瘤的早期研究中，我们报道了两个都删除了C mu基因，并且我们得出结论，这种缺失在正常分泌IgD的细胞中很常见。要了解在C delta 1外显子上游不存在开关区域的情况下如何发生这种开关重组，我们分离了另外七个BALB / c和两个分泌C57BL / 6 IgD的杂交瘤。我们确定了这些杂交瘤中的八个以及C57BL / 6杂交瘤B1-8中的开关重组连接的DNA序列。 Δ1和BALB / c，分泌IgD的浆细胞瘤TEPC1033。所有系在开关重组事件中均缺失了C mu基因，其中3条缺失了C delta 1外显子。 Δ开关重组连接序列类似于已发表的与其他重链基因5'发生的生产性开关重组的序列，这表明只要重链开关区域参与重组，就会利用非同源，非法的重组。

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