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首页> 外文期刊>Molecular and Cellular Biology >Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.
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Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.

机译：酿酒酵母包含与果蝇的主要热激诱导基因相关的复杂的多基因家族。

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Saccharomyces cerevisiae contains a family of genes related to the major heat shock-induced gene of Drosophila (hsp 70). Two members of the multigene family (YG100 and YG101) were isolated. The primary DNA sequences of more than one-half of the protein-encoding regions of YG100 and YG101 were determined and compared with the Drosophila hsp 70 gene sequence; the predicted amino acid sequences were 72 and 64% homologous to the sequence of the Drosophila hsp 70 protein, respectively. The predicted amino acid sequences of the yeast genes were 65% homologous. Our results demonstrate a striking sequence conservation of hsp 70-related sequences in evolution. Hybridization of the S. cerevisiae genes to total S. cerevisiae DNA indicated that the multigene family consists of approximately 10 members. Hybridization of labeled RNAs from heat-shocked and control cells suggested that, like transcription of the Drosophila hsp 70 gene, transcription of YG100 or a closely related gene is enhanced after heat shock. However, the amount of RNA sequences homologous to YG101 was reduced after heat shock. A multigene family related to the hsp 70 gene exists in Drosophila; transcription of some members is induced by heat shock, whereas transcription of others is not. Our results suggest that S. cerevisiae, like Drosophila, contains a multigene family of hsp 70-related sequences under complex transcriptional regulation and that the differential control, as well as the nucleotide sequence, has been highly conserved in evolution.
机译：酿酒酵母包含与果蝇主要热激诱导的基因相关的基因家族（hsp 70）。分离了多基因家族的两个成员（YG100和YG101）。确定了YG100和YG101蛋白质编码区一半以上的主要DNA序列，并将其与果蝇hsp 70基因序列进行了比较。预测的氨基酸序列与果蝇hsp 70蛋白的序列分别有72％和64％的同源性。酵母基因的预测氨基酸序列是65％同源的。我们的结果证明了进化中热休克蛋白70相关序列的惊人序列保守性。酿酒酵母基因与酿酒酵母DNA的杂交表明该多基因家族由大约10个成员组成。来自热休克细胞和对照细胞的标记RNA杂交表明，像果蝇hsp 70基因的转录一样，热激后，YG100或密切相关的基因的转录也会增强。然而，热激后与YG101同源的RNA序列的量减少了。果蝇中存在与hsp 70基因相关的多基因家族;一些成员的转录是由热激诱导的，而其他成员则不是。我们的结果表明，酿酒酵母像果蝇一样，在复杂的转录调控下含有hsp 70相关序列的多基因家族，并且差异控制以及核苷酸序列在进化中一直高度保守。

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