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首页> 外文期刊>Molecular and Cellular Biology >Topographical distribution of 5-methylcytosine in animal and plant DNA.
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Topographical distribution of 5-methylcytosine in animal and plant DNA.

机译：5-甲基胞嘧啶在动植物DNA中的地形分布。

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摘要

The topographical distribution of 5-methylcytosine on animal and plant cell DNA has been examined with methyl-sensitive restriction enzymes and gel electrophoresis analysis. These DNAs digested with the enzyme HpaII have a partially bimodal size distribution, indicating the existence of clusters of methylated and unmethylated CCGG sites in the DNA. By analyzing the methylation state of all CG moieties in restricted DNA fractions, it was possible to show that these genomes are, in general, arranged as clusters of relatively highly methylated and undermethylated regions. Plant DNA also contains 5-methylcytosine in the prototype sequence C-X-G. Restriction of this DNA with EcoRII revealed that these methyl groups are also distributed in clusters, suggesting that this is a general phenomenon. The undermethylated areas may correspond to the active fraction of the genome.
机译：用甲基敏感的限制性内切酶和凝胶电泳分析了5-甲基胞嘧啶在动物和植物细胞DNA上的形貌分布。用HpaII酶消化的这些DNA具有部分双峰大小分布，表明DNA中存在甲基化CCGG位和未甲基化CCGG位点的簇。通过分析限制性DNA片段中所有CG部分的甲基化状态，有可能表明这些基因组通常以甲基化程度较高和甲基化程度较低区域的簇的形式排列。植物DNA还包含原型序列为C-X-G的5-甲基胞嘧啶。用EcoRII对该DNA进行限制后发现，这些甲基也成簇分布，表明这是普遍现象。甲基化不足的区域可以对应于基因组的活性部分。

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