DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression.

P G Debenham; N Kartner; L Siminovitch; J R Riordan; V Ling

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DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression.

机译：DNA介导的多重耐药性转移和质膜糖蛋白表达。

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Colchicine-resistant Chinese hamster ovary (CHO) cell mutants whose resistance results from reduced drug permeability have been isolated previously in our laboratories. This reduced permeability affects a wide range of unrelated drugs, resulting in the mutants displaying a multiple drug resistance phenotype. A 170,000-dalton cell surface glycoprotein (P-glycoprotein) was identified, and its expression appears to correlate with the degree of resistance. In this study we were able to confer the multiple drug resistance phenotype on sensitive mouse L cells by DNA-mediated gene transfer of DNA obtained from the colchicine-resistant mutants. P-glycoprotein was detected in plasma membranes of these DNA transformants by staining with an antiserum raised against membranes of mutant CHO cells. These results are consistent with a causal relationship between P-glycoprotein expression and the multiple drug resistance phenotype.

机译：秋水仙碱抗性中国仓鼠卵巢（CHO）细胞突变体的耐药性是由降低的药物渗透性导致的，先前已在我们的实验室中分离出来。这种降低的通透性影响广泛的无关药物，导致突变体表现出多重耐药性表型。鉴定出一种170,000道尔顿的细胞表面糖蛋白（P-glycoprotein），其表达似乎与耐药程度相关。在这项研究中，我们能够通过从秋水仙碱抗性突变体获得的DNA的DNA介导的基因转移，在敏感的小鼠L细胞上赋予多重耐药性表型。通过用针对突变CHO细胞膜的抗血清染色，在这些DNA转化子的质膜中检测到P-糖蛋白。这些结果与P-糖蛋白表达和多重耐药性表型之间的因果关系一致。

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《Molecular and Cellular Biology》 |1982年第8期|共9页
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P G Debenham; N Kartner; L Siminovitch; J R Riordan; V Ling;
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中图分类细胞生物学;
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1. Co-amplification of double minute chromosomes, multiple drug resistance, and cell surface P-glycoprotein in DNA-mediated transformants of mouse cells. [J] . S M Robertson, V Ling, C P Stanners Molecular and Cellular Biology . 1984,第3期

机译：在小鼠细胞的DNA介导的转化子中，双分钟染色体，多重耐药性和细胞表面P-糖蛋白的共扩增。
2. Modulation of P-glycoprotein-mediated multidrug resistance by acceleration of passive drug permeation across the plasma membrane [J] . Regev R, Katzir H, Yeheskely-Hayon D, The FEBS journal . 2007,第23期

机译：通过加速跨膜质膜的被动药物渗透来调节P-糖蛋白介导的多药耐药性
3. A Transferable 20-Kilobase Multiple Drug Resistance-Conferring R Plasmid (pKL0018) from a Fish Pathogen (Lactococcus garvieae) Is Highly Homologous to a Conjugative Multiple Drug Resistance-Conferring Enterococcal Plasmid [J] . Takeshi Maki, Mudjekeewis D. Santos, Hidehiro Kondo, Applied and Environmental Microbiology . 2009,第10期

机译：来自鱼病原体（乳乳球菌）的可转移的20碱基多重耐药性R质粒（pKL0018）与共轭多重耐药性肠球菌质粒高度同源
4. Study of Drug Resistance in Clinical Isolates of Enterococci with special reference to high level aminoglycoside resistance, beta lactamase production and lateral transfer of drug resistance [C] . M.G. Karmarkar, Edwin S. Gershom, Santosh Kaul, Lancefield International Symposium on Streptococci and Streptococcal Diseases . 2006

机译：肠球菌临床分离株的耐药性研究，特别是高水平氨基糖苷抵抗，β内酰胺酶生产和耐药性横向转移
5. Biochemical characterization of NDR1, a plasma membrane-localized glycoprotein required for disease resistance in Arabidopsis thaliana. [D] . Coppinger, James Peter. 2005

机译：NDR1的生物化学特性，NDR1是拟南芥抗病所需的质膜定位糖蛋白。
6. DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression. [O] . P G Debenham, N Kartner, L Siminovitch, 1982

机译：DNA介导的多重耐药性转移和质膜糖蛋白表达。
7. DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression. [O] . Debenham, P G, Kartner, N, Siminovitch, L, 1982

机译：DNA介导的多重耐药性转移和质膜糖蛋白表达。
8. Characterization of Human Lysosomal Membrane Glycoproteins and Evidence for their Differentiation-Related Expression in the Plasma Membrane of Myelomonocytic Leukemia Cells [R] . Mane, S. M., Marzella, L., Bainton, D. F., 1988

机译：人溶酶体膜糖蛋白的表征及其在骨髓单核细胞白血病细胞质膜分化相关表达的证据

DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression.

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