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首页> 外文期刊>Molecular and Cellular Biology >Cytoplasmic and secreted Saccharomyces cerevisiae invertase mRNAs encoded by one gene can be differentially or coordinately regulated.
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Cytoplasmic and secreted Saccharomyces cerevisiae invertase mRNAs encoded by one gene can be differentially or coordinately regulated.

机译：由一个基因编码的细胞质和分泌的酿酒酵母转化酶mRNA可以有差异或协调性调控。

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A single structural gene, SUC2, encodes both secreted and cytoplasmic invertase in Saccharomyces cerevisiae. It is known that the unprocessed polypeptides which differ by a secretion signal sequence are encoded by separate mRNAs. This unusual transcriptional organization raises the question as to the degree to which the transcripts can be independently regulated. To define a system for studying this problem, we examined invertase transcription after various physiological perturbations of cells: rapid catabolite derepression, heat shock, and cell cycle arrest. With each treatment, fluctuations in mRNA levels for both cytoplasmic and secreted invertase were observed. We concluded that (i) catabolite-derepressed synthesis of the mRNAs occurs rapidly after a drop in glucose, is a sustained response, and does not require de novo protein synthesis; (ii) heat shock transcription of both invertase mRNAs is, in contrast, a brief and transient response requiring de novo protein synthesis; and (iii) alpha-mating hormone treatment (G1 phase arrest and release) results in regular and coordinated synthesis of both mRNAs midway between rounds of histone mRNA synthesis. We propose that invertase mRNA regulation involves constitutively synthesized transcriptional factors (observed during catabolite derepression) and transient factors (observed during heat shock and possibly during synchronous growth). Moreover, the mRNA levels for secreted and cytoplasmic invertase can be independently regulated.
机译：单个结构基因SUC2编码酿酒酵母中的分泌型和胞质转化酶。已知通过分泌信号序列不同的未加工的多肽由分开的mRNA编码。这种不寻常的转录组织提出了关于转录物可以被独立调节的程度的问题。为了定义研究此问题的系统，我们检查了细胞的各种生理扰动后的转化酶转录：快速分解代谢物抑制，热休克和细胞周期停滞。每次处理时，均观察到细胞质和分泌型转化酶的mRNA水平波动。我们得出的结论是：（i）葡萄糖下降后，分解代谢物降低的mRNA合成迅速发生，是持续的反应，不需要从头合成蛋白质; （ii）两种转化酶mRNA的热激转录相反，是短暂且短暂的反应，需要从头合成蛋白质; （iii）α-交配激素治疗（G1期阻滞和释放）可导致在两轮组蛋白mRNA合成之间的中途定期且​​协调地合成两种mRNA。我们建议，转化酶mRNA调控涉及组成性合成的转录因子（在分解代谢物阻抑过程中观察到）和瞬时因子（在热激过程中以及可能在同步生长过程中观察到）。此外，分泌的和胞质转化酶的mRNA水平可以独立调节。

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