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首页> 外文期刊>Molecular and Cellular Biology >Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.
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Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.

机译：非肌肉和肌肉原肌球蛋白同工型通过替代的RNA剪接和聚腺苷酸化作用从单个基因表达。

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The molecular basis for the expression of rat embryonic fibroblast tropomyosin 1 and skeletal muscle beta-tropomyosin was determined. cDNA clones encoding these tropomyosin isoforms exhibit complete identity except for two carboxy-proximal regions (amino acids 189 to 213 and 258 to 284) and different 3'-untranslated sequences. The isoform-specific regions delineate the troponin T-binding domains of skeletal muscle tropomyosin. Analysis of genomic clones indicates that there are two separate loci in the rat genome that contain sequences complementary to these mRNAs. One locus is a pseudogene. The other locus contains a single gene made up of 11 exons and spans approximately 10 kilobases. Sequences common to all mRNAs were found in exons 1 through 5 (amino acids 1 to 188) and exons 8 and 9 (amino acids 214 to 257). Exons 6 and 11 are specific for fibroblast mRNA (amino acids 189 to 213 and 258 to 284, respectively), while exons 7 and 10 are specific for skeletal muscle mRNA (amino acids 189 to 213 and 258 to 284, respectively). In addition, exons 10 and 11 each contain the entire 3'-untranslated sequences of the respective mRNAs including the polyadenylation site. Although the gene is also expressed in smooth muscle (stomach, uterus, and vas deferens), only the fibroblast-type splice products can be detected in these tissues. S1 and primer extension analyses indicate that all mRNAs expressed from this gene are transcribed from a single promoter. The promoter was found to contain G-C-rich sequences, a TATA-like sequence TTTTA, no identifiable CCAAT box, and two putative Sp1-binding sites.
机译：确定了大鼠胚胎成纤维细胞原肌球蛋白1和骨骼肌β-原肌球蛋白表达的分子基础。编码这些原肌球蛋白同工型的cDNA克隆除了两个羧基近端区域（氨基酸189-213和258-284）和不同的3'-非翻译序列外，具有完全的同一性。亚型特异性区域描绘了骨骼肌原肌球蛋白的肌钙蛋白T结合结构域。基因组克隆的分析表明，大鼠基因组中有两个单独的基因座，它们包含与这些mRNA互补的序列。一个基因座是假基因。另一个基因座包含一个由11个外显子组成的单一基因，跨度约10万个碱基。在外显子1至5（氨基酸1至188）以及外显子8和9（氨基酸214至257）中发现了所有mRNA共有的序列。外显子6和11对成纤维细胞mRNA具有特异性（分别为氨基酸189至213和258至284），而外显子7和10对骨骼肌mRNA具有特异性（分别为氨基酸189至213和258至284）。另外，外显子10和11各自包含包括聚腺苷酸化位点的各个mRNA的整个3'-非翻译序列。尽管该基因还在平滑肌（胃，子宫和输精管）中表达，但在这些组织中只能检测到成纤维细胞型剪接产物。 S1和引物延伸分析表明，从该基因表达的所有mRNA均从单个启动子转录而成。发现该启动子包含富含G-C的序列，TATA样序列TTTTA，无可识别的CCAAT盒和两个推定的Sp1结合位点。

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