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首页> 外文期刊>Molecular and Cellular Biology >A factor present in fetal calf serum enhances oncogene-induced transformation of rodent fibroblasts.
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A factor present in fetal calf serum enhances oncogene-induced transformation of rodent fibroblasts.

机译：胎牛血清中存在的一个因子会增强癌基因诱导的啮齿动物成纤维细胞转化。

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Our previous studies indicated that addition of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA) or teleocidin to Dulbecco modified Eagle medium supplemented with calf serum enhanced T24-induced focus formation in both the murine C3H 10T1/2 and rat 6 embryo fibroblast cell lines. In the present studies we have found that fetal calf serum (FCS) is more potent than 12-O-tetradecanoylphorbol-13-acetate in enhancing T24-induced focus formation, in terms of the number and size of the foci, in both C3H 10T1/2 and rat 6 cells. Time course studies indicate that FCS can exert this enhancing effect when it is added several days after the transfection with T24 DNA. In rat 6 cells, an 11-fold increase in T24-induced focus formation occurred when the transfected cultures were maintained for only 1 day in 5% FCS, starting 4 days after the transfection. Several known growth factors, including epidermal growth factor, transforming growth factors alpha and beta, insulin, and platelet-derived growth factor, did not enhance T24-induced transformation in these cell systems. Fractionation studies indicate that the factor present in FCS has a molecular weight of about 1,300, is not lipid soluble, and is acid, base, and heat stable. These findings suggest that a factor(s) normally present in serum may enhance the emergence of tumor cells in vivo, by acting in concert with an activated oncogene, during the multistage carcinogenic process.
机译：我们以前的研究表明，在补充有小牛血清的Dulbecco修饰的Eagle培养基中添加肿瘤启动子12-O-十四烷酰phorbol-13-乙酸盐（TPA）或Teleocidin可以增强T24诱导的鼠C3H 10T1 / 2和大鼠6的病灶形成。胚胎成纤维细胞系。在本研究中，我们发现在C3H 10T1中，就病灶的数量和大小而言，胎牛血清（FCS）在增强T24诱导的病灶形成方面比12-O-十四烷酰phorbol-13-乙酸盐更有效。 / 2和大鼠6个细胞。时程研究表明，当用T24 DNA转染数天后添加FCS时，它可以发挥这种增强作用。在大鼠6细胞中，从转染后4天开始，将转染的培养物在5％FCS中仅维持1天，T24诱导的焦点形成就会增加11倍。几种已知的生长因子，包括表皮生长因子，转化生长因子α和β，胰岛素和血小板衍生的生长因子，在这些细胞系统中并未增强T24诱导的转化。分馏研究表明，FCS中存在的因子分子量约为1,300，不溶于脂类，并且对酸，碱和热稳定。这些发现表明，在多阶段致癌过程中，血清中正常存在的一种因子可通过与活化的癌基因协同作用来增强体内肿瘤细胞的出现。

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