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首页> 外文期刊>Molecular and Cellular Biology >Yeast pheromone response pathway: characterization of a suppressor that restores mating to receptorless mutants.
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Yeast pheromone response pathway: characterization of a suppressor that restores mating to receptorless mutants.

机译：酵母信息素反应途径：恢复到无受体突变体交配的抑制剂的特征。

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Saccharomyces cerevisiae haploid cells, alpha and a, mate after being appropriately stimulated by the pheromone secreted by the opposite cell type (a-factor and alpha-factor, respectively). The binding of a pheromone to its receptor is a signal that initiates a series of intracellular changes that lead to the specific physiological alterations required for mating. To identify components of the signal transduction pathway, we sought pseudorevertants that restored mating competence to receptor mutants (MAT alpha ste3::LEU2). The suppressor srm1-1 was isolated as a recessive mutation that conferred temperature-sensitive growth to all strains and mating ability to MAT alpha ste3::LEU2 strains at the nonpermissive temperature. In addition, when srm1-1 mutants were shifted to the nonpermissive temperature, they exhibited two phenotypes characteristic of pheromone response, induction of FUS1 transcription and accumulation of cells in the G1 phase of the cell cycle. The srm1-1 mutation also suppressed a deletion of the alpha-factor-receptor gene in a cells. Together, these phenotypes suggest that the wild-type SRM1 product is a component of the pheromone response pathway. Deletion of STE4 or STE5, which are required in both haploid cell types for mating and response to pheromone, was not suppressed by srm1-1, suggesting that the SRM1 product may function before the STE4 and STE5 products. SRM1 is an essential gene and is expressed in both haploid cell types as well as in the product of their mating, a/alpha diploids. Homozygous srm1-1 a/alpha diploids were temperature sensitive although they did not arrest in G1. Thus, the SRM1 product may also have a role in the vegetative life cycle of cells.
机译：酿酒酵母单倍体细胞α和α在受到相反细胞类型（分别为α因子和α因子）分泌的信息素的适当刺激后交配。信息素与其受体的结合是引发一系列细胞内变化的信号，该变化导致交配所需的特定生理学改变。为了确定信号转导途径的组成部分，我们寻求伪逆转体，以恢复与受体突变体（MAT alpha ste3 :: LEU2）的交配能力。抑制子srm1-1作为隐性突变被分离出来，该突变赋予所有菌株温度敏感的生长，并在非允许温度下使其与MAT alpha ste3 :: LEU2菌株的交配能力。此外，当srm1-1突变体转移到非容许温度时，它们表现出两种信息素特征表型，即信息素应答，FUS1转录诱导和细胞周期G1期细胞蓄积。 srm1-1突变还抑制了细胞中α因子受体基因的缺失。这些表型共同表明，野生型SRM1产物是信息素反应途径的组成部分。在单倍体细胞类型中，交配和对信息素的应答都需要删除STE4或STE5，而srm1-1并未抑制它的删除，这表明SRM1产物可能先于STE4和STE5产物起作用。 SRM1是必不可少的基因，在单倍体细胞类型及其交配的a / alpha二倍体产物中均有表达。纯合子srm1-1 a / alpha二倍体对温度敏感，尽管它们没有停留在G1中。因此，SRM1产品也可能在细胞的营养生命周期中起作用。

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