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首页> 外文期刊>Molecular and Cellular Biology >The Drosophila even-skipped promoter is transcribed in a stage-specific manner in vitro and contains multiple, overlapping factor-binding sites.
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The Drosophila even-skipped promoter is transcribed in a stage-specific manner in vitro and contains multiple, overlapping factor-binding sites.

机译：果蝇均匀跳过的启动子在体外以阶段特异性方式转录，并包含多个重叠的因子结合位点。

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摘要

To investigate the factors contributing to regulation of expression of the Drosophila segmentation gene even-skipped (eve), we have analyzed both the in vitro transcription and eve-promoter-binding proteins in embryo extracts. We show that the eve promoter is accurately and efficiently expressed in nuclear extracts derived from Drosophila embryos and that transcription is more efficient in extracts prepared from embryos at early stages of development than in those from older embryos, broadly reproducing the temporal pattern of expression observed in vivo. This stage-specific expression is dependent on sequences upstream of the eve transcription start site which contain multiple binding sites for at least two distinct proteins present in embryo nuclei. One of these proteins, the binding sites for which correspond to the sequences required for stage-specific expression, appears to be the previously described GAGA factor. Although the binding activity of the GAGA factor remains constant, the level of the binding activity of the other protein, which we have called the TCCT factor, changes during the course of embryogenesis. Activity is first detected 3 to 5 h after fertilization and decreases during later stages of embryogenesis. We discuss the possibility that the TCCT factor plays a role in the maintenance or refinement of the eve expression pattern.
机译：为了研究影响果蝇分割基因表达的调控因素，我们分析了胚胎提取物中的体外转录和前体启动子结合蛋白。我们表明，eve启动子在果蝇胚胎核提取物中得到了准确有效的表达，转录在早期发育阶段从胚胎中制备的提取物中比老龄胚胎中的转录更有效，广泛地再现了在果蝇中观察到的时间表达模式。体内。该阶段特异性表达取决于前夜转录起始位点上游的序列，该序列含有胚核中存在的至少两种不同蛋白质的多个结合位点。这些蛋白质之一，其结合位点对应于阶段特异性表达所需的序列，似乎是先前描述的GAGA因子。尽管GAGA因子的结合活性保持恒定，但在胚胎发生过程中，我们称为TCCT因子的其他蛋白质的结合活性水平却发生了变化。受精后3至5小时首先检测到活性，并在胚胎发生的后期降低。我们讨论了TCCT因子在前夕表达模式的维持或完善中发挥作用的可能性。

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