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首页> 外文期刊>Molecular and Cellular Biology >Two types of TATA elements for the CYC1 gene of the yeast Saccharomyces cerevisiae.
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Two types of TATA elements for the CYC1 gene of the yeast Saccharomyces cerevisiae.

机译：酿酒酵母中CYC1基因的两种TATA元素。

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Functional TATA elements in the 5' untranslated region of the CYC1 gene in the yeast Saccharomyces cerevisiae have been defined by transcriptional analysis of site-directed mutations. Five sites previously suggested to contain functional TATA elements were altered individually and in all possible combinations. The results indicated that only two elements are required for transcription at the normal level and the normal start sites. The two functional TATA elements are located at sites -178 and -123, where the A of the ATG start codon is assigned nucleotide position +1. They direct initiation within windows encompassing -70 to -46 and -46 to -28, respectively. Only when both of the upstream TATA sites were rendered nonfunctional were the third and fourth downstream TATA-like sequences activated, as indicated by the presence of low levels of transcription starting at -28. The two upstream functional TATA elements differed in sequence. The sequence of the most 5' one at site 1, denoted beta-type, was ATATATATAT, whereas that of the second one at site 2, denoted alpha-type, was TATATAAAA. The following rearrangements of the beta-type and alpha-type elements at two sites (1 and 2) were examined: site1 beta-site2 alpha; site 1 alpha-site 2 beta; site1 alpha-site2 alpha; and site1 beta-site2 beta. When different types were at different sites (site1 beta-site2 alpha and site1 alpha-site2 beta), both were used equally. In contrast, when the same type was present at both sites (site1 alpha-site2 alpha and site1 beta-site2 beta), only the upstream element was used. We suggest that the two TATA elements are recognized by different factors of the transcription apparatus.
机译：通过定点突变的转录分析，已经确定了酿酒酵母中CYC1基因5'非翻译区中的功能性TATA元件。先前建议包含功能性TATA元素的五个位点已被单独更改，并以所有可能的组合更改。结果表明，在正常水平和正常起始位点转录仅需要两个元件。这两个功能性TATA元件位于位点-178和-123，其中ATG起始密码子的A被指定为核苷酸位置+1。它们在分别包含-70至-46和-46至-28的窗口内引导启动。仅当两个上游TATA位点都失去功能时，第三和第四个下游TATA样序列才被激活，如从-28开始的低水平转录的存在所表明的。两个上游功能性TATA元件的顺序不同。位点1处最5'端的序列表示为β型，而位点2处第二个5'端的序列表示为α型，是TATATAAAA。检查了两个位点（1和2）的β型和α型元素的下列重排：site1 beta-site2 alpha;网站1 alpha网站2 beta; site1 alpha-site2 alpha;和site1 beta-site2 beta。当不同的类型位于不同的站点（site1 beta-site2 alpha和site1 alpha-site2 beta）时，两者均被平等使用。相反，当两个站点（site1 alpha-site2 alpha和site1 beta-site2 beta）都存在相同类型时，仅使用上游元素。我们建议两个TATA元素被转录设备的不同因素识别。

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