Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ.

P Cortes; O Flores; D Reinberg

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Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ.

机译：哺乳动物RNA聚合酶II参与特异性转录的因素：转录因子IIA的纯化和分析以及转录因子IIJ的鉴定。

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The previously described transcription factor IIA (TFIIA) protein fraction was separated into two factors that affect transcription, TFIIA and TFIIJ. TFIIA was found to have a stimulatory effect, and TFIIJ was found to be required for transcription. The requirement of TFIIJ was observed when bacterially produced purified human or yeast (Saccharomyces cerevisiae) TATA-binding protein (TBP) was used in lieu of the endogenous HeLa cell TFIID complex, suggesting that TFIIJ may be part of the TFIID complex. The stimulatory activity of TFIIA was found also to be dependent on the source of the TBP. Transcription reactions reconstituted with TFIID were stimulated by TFIIA; however, when human or yeast TBP was used instead of TFIID, TFIIA had no effect. TFIIA was found to interact with the TBP and was extensively purified by the use of affinity chromatography on columns containing immobilized recombinant yeast TBP. TFIIA is a heterotrimer composed of polypeptides of 34, 19, and 14 kDa. These three polypeptides were required to isolate, by using the gel mobility shift assay, a stable complex between TBP and the TATA box sequence.

机译：先前描述的转录因子IIA（TFIIA）蛋白部分被分成两个影响转录的因子TFIIA和TFIIJ。发现TFIIA具有刺激作用，并且发现转录需要TFIIJ。当使用细菌生产的纯化的人或酵母（酿酒酵母）TATA结合蛋白（TBP）代替内源性HeLa细胞TFIID复合物时，观察到TFIIJ的需要，这表明TFIIJ可能是TFIID复合物的一部分。还发现TFIIA的刺激活性也取决于TBP的来源。 TFIIA刺激了用TFIID重建的转录反应;但是，当使用人或酵母TBP代替TFIID时，TFIAA无效。发现TFIAA与TBP相互作用，并且通过在包含固定的重组酵母TBP的柱上使用亲和色谱法被广泛纯化。 TFIIA是由34、19和14kDa的多肽组成的异源三聚体。通过使用凝胶迁移率移动分析，需要这三种多肽来分离TBP和TATA盒序列之间的稳定复合物。

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《Molecular and Cellular Biology》 |1992年第1期|共9页
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P Cortes; O Flores; D Reinberg;
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中图分类细胞生物学;
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1. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex. [J] . E Maldonado, I Ha, P Cortes, Molecular and Cellular Biology . 1990,第12期

机译：哺乳动物RNA聚合酶II参与特异性转录的因素：转录因子IIA，IID和IIB在形成具有转录能力的复合物期间的作用。
2. Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID. [J] . N Nakajima, M Horikoshi, R G Roeder Molecular and Cellular Biology . 1988,第10期

机译：哺乳动物RNA聚合酶II参与特异性转录的因素：TFIID的纯化，遗传特异性和TATA盒启动子相互作用。
3. TATA box-binding protein (TBP) is a constituent of the polymerase I-specific transcription initiation factor TIF-IB (SL1) bound to the rRNA promoter and shows differential sensitivity to TBP-directed reagents in polymerase I, II, and III transcription factors. [J] . C A Radebaugh, J L Matthews, G K Geiss, Molecular and Cellular Biology . 1994,第1期

机译：TATA盒结合蛋白（TBP）是与rRNA启动子结合的聚合酶I特异性转录起始因子TIF-IB（SL1）的组成部分，在聚合酶I，II和III转录因子中对TBP定向试剂表现出不同的敏感性。
4. Atypical Transcription Factors Function with RNA Polymerase II in Trypanosomes to Produce the Spliced Leader RNA [C] . V. Bellofatto, J.B. Palenchar, A. Da International Congress of Parasitology . 2006

机译：非典型转录因子在锥虫中具有RNA聚合酶II的功能，以产生剪接前导RNA
5. Identification of factors regulating transcription termination by RNA polymerase II in Saccharomyces cerevisiae. [D] . Nedea, Eduard. 2009

机译：鉴定酿酒酵母中RNA聚合酶II调控转录终止的因素。
6. Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ. [O] . P Cortes, O Flores, D Reinberg 1992

机译：哺乳动物RNA聚合酶II参与特异性转录的因素：转录因子IIA的纯化和分析以及转录因子IIJ的鉴定。
7. Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ. [O] . Cortes, P, Flores, O, Reinberg, D 1992

机译：哺乳动物RNA聚合酶II参与特异性转录的因素：转录因子IIA的纯化和分析以及转录因子IIJ的鉴定。

Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ.

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