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首页> 外文期刊>Molecular and Cellular Biology >Test for temporal or spatial restrictions in gene product function during the cell division cycle.
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Test for temporal or spatial restrictions in gene product function during the cell division cycle.

机译：测试细胞分裂周期中基因产物功能的时间或空间限制。

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The ability of a functional gene to complement a nonfunctional gene may depend upon the intracellular relationship of the two genes. If so, the function of the gene product in question must be limited in time or in space. CDC (cell division cycle) gene products of Saccharomyces cerevisiae control discrete steps in cell division; therefore, they constitute reasonable candidates for genes that function with temporal or spatial restrictions. In an attempt to reveal such restrictions, we compared the ability of a CDC gene to complement a temperature-sensitive cdc gene in diploids where the genes are located within the same nucleus to complementation in heterokaryons where the genes are located in different nuclei. In CDC X cdc matings, complementation was monitored in rare heterokaryons by assaying the production of cdc haploid progeny (cytoductants) at the restrictive temperature. The production of cdc cytoductants indicates that the cdc nucleus was able to complete cell division at the restrictive temperature and implies that the CDC gene product was provided by the other nucleus or by cytoplasm in the heterokaryon. Cytoductants from cdc28 or cdc37 crosses were not efficiently produced, suggesting that these two genes are restricted spatially or temporally in their function. We found that of the cdc mutants tested 33 were complemented; cdc cytoductants were recovered at least as frequently as CDC cytoductants. A particularly interesting example was provided by the CDC4 gene. Mutations in CDC4 were found previously to produce a defect in both cell division and karyogamy. Surprisingly, the cell division defect of cdc4 nuclei is complemented by CDC4 nuclei in a heterokaryon, whereas the karyogamy defect is not.
机译：功能基因补充非功能基因的能力可能取决于两个基因的细胞内关系。如果是这样，所讨论的基因产物的功能必须在时间或空间上受到限制。酿酒酵母的CDC（细胞分裂周期）基因产物控制细胞分裂的离散步骤;因此，它们构成了具有时间或空间限制的基因的合理候选者。为了揭示这种限制，我们比较了CDC基因在基因位于同一核内的二倍体中与温度敏感的cdc基因互补的能力与在基因位于不同核中的异核体中互补的能力。在CDC X cdc交配中，通过检测限制性温度下cdc单倍体后代（细胞生成剂）的产生来监测稀有异核体中的互补。 CDC细胞生成剂的产生表明CDC核能够在限制性温度下完成细胞分裂，并暗示CDC基因产物是由另一个核或异核体中的细胞质提供的。没有有效地产生来自cdc28或cdc37杂交的细胞周期调节剂，表明这两个基因的功能在空间或时间上受到限制。我们发现在测试的cdc突变体中有33个互补。 cdc细胞生成素的回收率至少与CDC细胞生成素的回收率相同。 CDC4基因提供了一个特别有趣的例子。先前发现CDC4的突变会在细胞分裂和核分裂中产生缺陷。令人惊讶的是，异核体中cdc4核可补充cdc4核的细胞分裂缺陷，而核配体缺陷则无。

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