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首页> 外文期刊>Molecular and Cellular Biology >Biochemical evidence for multiple independent emetine resistance genes in Chinese hamster cells.
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Biochemical evidence for multiple independent emetine resistance genes in Chinese hamster cells.

机译：中国仓鼠细胞中多个独立的依米丁耐药基因的生化证据。

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摘要

Hybridization-complementation studies indicated that mutations in multiple genes can render Chinese hamster cells resistant to the alkaloid translation inhibitor emetine. Two of the genes, emtA and emtB, recognized in Chinese hamster lung and ovary cell lines, respectively, are known to affect the ribosomes of the cells directly. Although mutations in a third gene, emtC, affect the translation apparatus of Chinese hamster peritoneal cells in vitro (Wasmuth et al., Mol. Cell. Biol. 1:58-65, 1981), the molecular product of the emtC locus remains to be determined. To study the molecular basis for genetic complementation among emetine-resistant Chinese hamster cell mutants, we analyzed ribosomal proteins elaborated by complementing, emetine-sensitive hybrid clones (EmtB X EmtA and EmtB X EmtC) and by emetine-resistant clones that segregated from the hybrids. The electrophoretic forms of ribosomal protein S14 (the emtB gene product) elaborated by these clones indicated that the EmtA and EmtC phenotypes are independent of the emtB locus and that the emtA and emtC loci are not chromosomally linked to emtB.
机译：杂交互补研究表明，多个基因的突变可使中国仓鼠细胞对生物碱翻译抑制剂依米丁产生抗性。已知分别在中国仓鼠肺和卵巢细胞系中识别的两个基因emtA和emtB直接影响细胞的核糖体。尽管第三个基因emtC的突变会在体外影响中国仓鼠腹膜细胞的翻译设备（Wasmuth等人，Mol。Cell。Biol。1：58-65，1981），但emtC基因座的分子产物仍然存在被确定。为了研究抗metetine的中国仓鼠细胞突变体之间遗传互补的分子基础，我们分析了由互补的emetine敏感的杂种克隆（EmtB X EmtA和EmtB X EmtC）以及从杂种中分离出来的具有emetine的克隆精心制作的核糖体蛋白。这些克隆阐述的核糖体蛋白S14（emtB基因产物）的电泳形式表明EmtA和EmtC表型独立于emtB基因座，并且emtA和emtC基因座未与emtB染色体连接。

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