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首页> 外文期刊>Molecular and Cellular Biology >Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2.
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Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2.

机译：鉴定介导酿酒酵母烯醇酶基因ENO2的葡萄糖依赖性诱导的调节区。

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There are two yeast enolase genes, designated ENO1 and ENO2, which are expressed differentially in vegetative cells grown on glucose and in cells grown on gluconeogenic carbon sources. ENO2 is induced more than 20-fold in cells grown on glucose, whereas ENO1 expression is similar in cells grown on glucose and in cells grown on gluconeogenic carbon sources. Sequences within the 5' flanking region of ENO2 which are required for glucose-dependent induction were identified by deletion mapping analysis. These studies were carried out by using a fused gene containing the ENO2 5' flanking sequences and the ENO1 coding sequences. This fused gene undergoes glucose-dependent induction and is expressed at the same level as the resident ENO2 gene in cells grown on glucose or gluconeogenic carbon sources. Expression of fused genes containing deletion mutations within the ENO2 5' flanking region was monitored after integration at the ENO1 locus of a strain carrying a deletion of the resident ENO1 coding sequences. This analysis showed that there are two upstream activation sites located immediately upstream and downstream from a position 461 base pairs upstream from the transcriptional initiation site. Either one of these upstream activation sites is sufficient for glucose-dependent induction and normal gene expression in the presence of gluconeogenic carbon sources. Deletion of both regulatory regions results in a complete loss of gene expression. The regulatory regions function normally in both orientations relative to the coding sequences. Mutant fused genes containing small deletions within the regulatory regions were constructed; these genes were expressed normally in gluconeogenic carbon sources but were not induced in the presence of glucose. Based on this analysis, ENO2 contains a cis-acting regulatory region which is required for gene expression and mediates glucose-dependent induction of gene expression.
机译：有两个酵母烯醇酶基因，分别命名为ENO1和ENO2，它们在葡萄糖生长的营养细胞和葡萄糖异生碳源生长的细胞中差异表达。 ENO2在葡萄糖上生长的细胞中被诱导超过20倍，而ENO1在葡萄糖上生长的细胞和在糖异生碳源上生长的细胞中的表达相似。通过缺失作图分析鉴定了葡萄糖依赖性诱导所需的ENO 2的5'侧翼区域内的序列。这些研究是通过使用包含ENO2 5'侧翼序列和ENO1编码序列的融合基因进行的。该融合基因经历葡萄糖依赖性诱导，并在葡萄糖或糖异生碳源上生长的细胞中以与驻留ENO2基因相同的水平表达。在携带有驻留的ENO1编码序列缺失的菌株的ENO1基因座处整合后，监测在ENO2 5'侧翼区域内包含缺失突变的融合基因的表达。该分析表明，在转录起始位点上游461个碱基对的上游和下游有两个上游激活位点。在存在糖异生碳源的情况下，这些上游激活位点之一足以进行葡萄糖依赖性诱导和正常基因表达。两个调节区的缺失导致基因表达的完全丧失。调节区通常在相对于编码序列的两个方向上起作用。构建了在调控区内含有小缺失的突变融合基因;这些基因在糖异生碳源中正常表达，但在葡萄糖存在下不被诱导。基于此分析，ENO2包含基因表达所需的顺式作用调节区，并介导葡萄糖依赖性基因表达的诱导。

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