• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Rapid cytoplasmic turnover of c-myc mRNA: requirement of the 3' untranslated sequences.
【24h】

Rapid cytoplasmic turnover of c-myc mRNA: requirement of the 3' untranslated sequences.

机译：c-myc mRNA的快速胞质更新：3'非翻译序列的要求。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Expression of the c-myc gene can be controlled by transcriptional or posttranscriptional mechanisms (or both), depending on the cell type and the growth conditions. An important mechanism of posttranscriptional regulation is modulation of cytoplasmic c-myc mRNA stability; normal human and murine c-myc mRNAs have cytoplasmic half-lives of 30 min or less. To elucidate the c-myc sequences which impart this unusually high rate of cytoplasmic transcript turnover, we have constructed various deletion and hybrid c-myc genes and analyzed the cytoplasmic stability of the mRNAs produced from them in stably transfected murine fibroblasts. The results indicate that sequences contained within the 5' and 3' ends of the c-myc transcript can affect cytoplasmic stability. Specifically, the 3' untranslated sequences of c-myc exon 3 are required for, but do not ensure, a high rate of transcript turnover in the cytoplasm. Exon 2 coding sequences do not seem to be involved, and exon 1 sequences at the 5' end of the transcript have only a small effect on cytoplasmic transcript stability. The sequences that are primarily responsible for the short c-myc RNA half-life were localized to a region of 140 bases in the 3' untranslated region.
机译：取决于细胞类型和生长条件，可以通过转录或转录后机制（或两者）控制c-myc基因的表达。转录后调控的重要机制是调节细胞质c-myc mRNA的稳定性。正常人和鼠c-myc mRNA的细胞质半衰期为30分钟或更短。为了阐明赋予这种异常高的胞质转录更新率的c-myc序列，我们构建了各种缺失和杂合c-myc基因，并分析了它们在稳定转染的鼠成纤维细胞中产生的mRNA的胞质稳定性。结果表明，c-myc转录本的5'和3'末端包含的序列可以影响细胞质的稳定性。具体而言，c-myc外显子3的3'非翻译序列是必需的，但不能确保细胞质中转录物的高转换率。似乎不涉及外显子2编码序列，并且在转录本5'端的外显子1序列对细胞质转录本的稳定性影响很小。主要负责短c-myc RNA半衰期的序列位于3'非翻译区的140个碱基的区域。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号