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首页> 外文期刊>Molecular and Cellular Biology >Genomic organization and sequence of the Gus-s alpha allele of the murine beta-glucuronidase gene.
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Genomic organization and sequence of the Gus-s alpha allele of the murine beta-glucuronidase gene.

机译：鼠β-葡糖醛酸糖苷酶基因的Gus-sα等位基因的基因组组织和序列。

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摘要

The Gus-s alpha allele of the mouse beta-glucuronidase gene exhibits a high degree of inducibility by androgens due to its linkage with the Gus-r alpha regulatory locus. We isolated Gus-s alpha on a 28-kilobase pair fragment of mouse chromosome 5 and found that it contains 12 exons and 11 intervening sequences spanning 14 kilobase pairs of this genomic segment. The mRNA cap site was identified by ribonuclease protection and primer extension analyses which revealed an unusually short 5' noncoding sequence of 12 nucleotides. Proximal regulatory sequences in the 5'-flanking DNA and the complete sequence of the Gus-s alpha mRNA transcript were also determined. Comparison of the amino acid sequence determined from the Gus-s alpha nucleotide sequence with that of human beta-glucuronidase indicated that the two human mRNA species differ due to alternate splicing of an exon homologous to exon 6 of the mouse gene.
机译：小鼠β-葡糖醛酸糖苷酶基因的Gus-sα等位基因由于与Gus-r alpha调控基因座相连，因此具有雄激素的高度诱导性。我们在小鼠染色体5的28碱基对片段上分离了Gus-s alpha，发现它包含12个外显子和11个中间序列，跨越该基因组片段的14个碱基对。通过核糖核酸酶保护和引物延伸分析鉴定了mRNA帽位点，其揭示了异常短的12个核苷酸的5'非编码序列。还确定了5'侧翼DNA中的近端调控序列和Gus-sαmRNA转录物的完整序列。由Gus-sα核苷酸序列确定的氨基酸序列与人β-葡糖醛酸糖苷酶的氨基酸序列的比较表明，由于与小鼠基因的外显子6同源的外显子的可变剪接，两个人mRNA种类不同。

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