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首页> 外文期刊>Molecular and Cellular Biology >Splicing of a yeast intron containing an unusual 5' junction sequence.
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Splicing of a yeast intron containing an unusual 5' junction sequence.

机译：包含异常5'连接序列的酵母内含子的剪接。

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The Saccharomyces cerevisiae COX5b gene contains a small intron that is unique in two respects. First, it interrupts the ATG codon that initiates translation of the COX5b product. Second, it contains a sequence at the 5' splice junction (5'-GCATGT-3') that differs from the highly conserved yeast hexanucleotide (5'-GTAPyGT-3') and from the 5'-GT found at the corresponding position in nearly all introns of eucaryotic protein-coding genes. We have analyzed both the transcripts derived from the COX5b gene and the splicing of its intron. We show here that an unspliced mRNA precursor constituted a minor fraction of the total COX5b message, even when the gene was overexpressed. We also show that both major transcripts derived from COX5b had been spliced. Our results suggest that at least in the case of COX5b, a 5'-GC can function as efficiently as the highly conserved 5'-GT in the splicing reaction.
机译：酿酒酵母COX5b基因包含一个小内含子，在两个方面都是唯一的。首先，它会中断ATG密码子，从而启动COX5b产品的翻译。其次，它在5'剪接点（5'-GCATGT-3'）包含一个序列，该序列不同于高度保守的酵母六核苷酸（5'-GTAPyGT-3'）和在相应位置发现的5'-GT在几乎所有的真核蛋白质编码基因的内含子中。我们已经分析了来自COX5b基因的转录本及其内含子的剪接。我们在这里显示，即使基因过表达，未剪接的mRNA前体也占总COX5b信息的一小部分。我们还显示源自COX5b的两个主要转录本均已剪接。我们的结果表明，至少在COX5b的情况下，5'-GC可以与剪接反应中高度保守的5'-GT一样有效。

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