Maintenance of cellular proliferation by adenovirus early region 1A in fibroblasts conditionally immortalized by using simian virus 40 large T antigen requires conserved region 1.

T E Riley; A Follin; N C Jones; P S Jat

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Maintenance of cellular proliferation by adenovirus early region 1A in fibroblasts conditionally immortalized by using simian virus 40 large T antigen requires conserved region 1.

机译：通过使用猿猴病毒40大T抗原有条件永生的成纤维细胞中腺病毒早期区域1A维持细胞增殖需要保守的区域1。

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Various mutants of adenovirus E1A were assayed for their ability to complement the growth defect at the nonpermissive temperature for the cell line tsa14 which was isolated by immortalizing rat embryo fibroblasts with the thermolabile large T antigen of tsA58. This cell line grows indefinitely at the permissive temperature but undergoes rapid growth arrest upon shift up to the nonpermissive temperature. Since this growth arrest can be overcome by introduction of wild-type simian virus 40 large T antigen, human papillomavirus 16 E7, and adenovirus E1A, the tsa14 cells provided an excellent system for defining regions of E1A necessary for complementation of the growth defect. We demonstrate that conserved region 1 (CR1) is the region of E1A required for complementation. While CR2 of E1A has been shown to be required for the immortalization of primary cells and is also necessary for the binding of the 105-kDa retinoblastoma protein, mutations within this region did not abrogate complementation of the growth defect. However, since both CR1 and CR2 have previously been shown to be absolutely required for immortalization of primary cells by adenovirus E1A, this evidence suggests that the tsa14 system assays for the maintenance of proliferation and that this requires CR1.

机译：分析了腺病毒E1A的各种突变体在非容许温度下对细胞系tsa14补充生长缺陷的能力，所述细胞系通过用热不稳定的tsA58大T抗原使大鼠胚胎成纤维细胞永生化而分离。该细胞系在容许温度下无限期生长，但在转移至非容许温度时会经历快速的生长停滞。由于可以通过引入野生型猿猴病毒40大T抗原，人乳头瘤病毒16 E7和腺病毒E1A来克服这种生长停滞，因此tsa14细胞提供了一个极好的系统，用于定义补充生长缺陷所必需的E1A区域。我们证明保守区1（CR1）是E1A互补所需的区域。虽然已显示E1A的CR2是永生化永生细胞所必需的，也是结合105 kDa视网膜母细胞瘤蛋白所必需的，但该区域内的突变并未消除生长缺陷的互补。但是，由于先前已经证明，腺病毒E1A永生化原代细胞绝对需要CR1和CR2，因此该证据表明tsa14系统测定了维持增殖，因此需要CR1。

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《Molecular and Cellular Biology》 |1990年第12期|共10页
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T E Riley; A Follin; N C Jones; P S Jat;
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中图分类细胞生物学;
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1. Two regions of simian virus 40 large T-antigen independently extend the life span of primary C57BL/6 mouse embryo fibroblasts and cooperate in immortalization. [J] . Tevethia MJ, Lacko HA, Conn A Virology . 1998,第2期

机译：猿猴病毒40个大T抗原的两个区域独立地延长了原代C57BL / 6小鼠胚胎成纤维细胞的寿命，并在永生化方面协同作用。
2. Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses VIII. Association of Simian Virus 40 Transplantation Antigen with a Specific Region of the Early Viral Genome [J] . Andrew M. Lewis, Wallace P. Rowe Journal of Virology . 1973,第4期

机译：NondefectiveAdenovirus 2-咪木病毒40杂交病毒的研究。猿猴病毒40移植抗原与早期病毒基因组特定区域的联系
3. Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses VIII. Association of Simian Virus 40 Transplantation Antigen with a Specific Region of the Early Viral Genome [J] . Andrew M. Lewis, Wallace P. Rowe Journal of Virology . 1973,第4期

机译：NondefectiveAdenovirus 2-咪木病毒40杂交病毒的研究。猿猴病毒40移植抗原与早期病毒基因组特定区域的联系
4. The DNA damage response of rodent fibroblasts expressing all or part of simian virus 40 large T antigen. [D] . Cole, Sara Lynn. 2001

机译：表达全部或部分猿猴病毒40大T抗原的啮齿动物成纤维细胞的DNA损伤反应。
5. Maintenance of cellular proliferation by adenovirus early region 1A in fibroblasts conditionally immortalized by using simian virus 40 large T antigen requires conserved region 1. [O] . T E Riley, A Follin, N C Jones, 1990

机译：通过使用猿猴病毒40大T抗原有条件地永生的成纤维细胞中腺病毒早期区域1A维持细胞增殖需要保守的区域1。
6. Maintenance of cellular proliferation by adenovirus early region 1A in fibroblasts conditionally immortalized by using simian virus 40 large T antigen requires conserved region 1. [O] . Riley, T E, Follin, A, Jones, N C, 1990

机译：通过使用猿猴病毒40大T抗原有条件地永生的成纤维细胞中腺病毒早期区域1A维持细胞增殖需要保守的区域1。

Maintenance of cellular proliferation by adenovirus early region 1A in fibroblasts conditionally immortalized by using simian virus 40 large T antigen requires conserved region 1.

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