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首页> 外文期刊>Molecular and Cellular Biology >SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.
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SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

机译：Disctyostelium discoideum中的SAS1和SAS2，GTP结合蛋白基因与酿酒酵母中的必需基因具有序列相似性。

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We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be involved in interaction with guanine nucleotides. The protein sizes, spacings of GTP-binding domains, and carboxyl-terminal sequences suggest their relationship to the ubiquitous ras-type proteins. Their sequences, however, are sufficiently different to indicate that they are not true ras proteins. More extensive sequence identity (approximately 55%) is shared with the YPT1 and SEC4 proteins from Saccharomyces cerevisiae. These yeast proteins are essential for growth and are believed to be involved in intracellular signaling associated with membrane function. SAS1 and SAS2 exhibit distinct patterns of genomic organization and developmentally regulated gene expression. SAS1 contains introns and is associated with a developmentally regulated repetitive element. SAS2 is colinear with its mRNA and does not appear to be closely linked with this repetitive element. Both genes are expressed during growth and throughout development. SAS1 is maximally expressed during cytodifferentiation, when two sizes of SAS1 mRNA are detectable. SAS2 mRNA levels are maximal during culmination. On the basis of the expression patterns of the SAS genes and their relationship to the YPT1 and SEC4 genes, we discuss possible functions of the SAS proteins.
机译：我们已经从Disctyostelium discoideum中鉴定了两个新的，密切相关的基因SAS1和SAS2。它们编码约20千克的小蛋白质，其氨基酸序列被认为与鸟嘌呤核苷酸相互作用。蛋白质的大小，GTP结合结构域的间隔以及羧基末端序列表明它们与普遍存在的ras型蛋白质的关系。但是，它们的序列差异很大，表明它们不是真正的ras蛋白。与来自酿酒酵母的YPT1和SEC4蛋白具有更广泛的序列同一性（约55％）。这些酵母蛋白对于生长是必不可少的，并且被认为参与与膜功能相关的细胞内信号传导。 SAS1和SAS2展示了不同的基因组组织模式和受发育调控的基因表达。 SAS1包含内含子，并与发育受调控的重复元件相关。 SAS2与它的mRNA共线，并且似乎与该重复元件没有紧密联系。这两个基因在生长期间和整个发育过程中均表达。当可检测到两种大小的SAS1 mRNA时，SAS1在细胞分化过程中最大程度地表达。最高点时，SAS2 mRNA水平最高。基于SAS基因的表达方式及其与YPT1和SEC4基因的关系，我们讨论了SAS蛋白的可能功能。

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